4.7 Article

Chemotaxis-driven assembly of endothelial barrier in a tumor-on-a-chip platform

期刊

LAB ON A CHIP
卷 16, 期 10, 页码 1886-1898

出版社

ROYAL SOC CHEMISTRY
DOI: 10.1039/c6lc00184j

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资金

  1. National Institute of Arthritis and Musculoskeletal and Skin Diseases of the National Institutes of Health [R01 AR063184-02]
  2. ARCS foundation
  3. Ruth L. Kirschstein National Research Service Award NIH/NHLBI [T32 HL 105373]
  4. [NS047101]
  5. NATIONAL HEART, LUNG, AND BLOOD INSTITUTE [T32HL105373] Funding Source: NIH RePORTER
  6. NATIONAL INSTITUTE OF ARTHRITIS AND MUSCULOSKELETAL AND SKIN DISEASES [R01AR063184] Funding Source: NIH RePORTER
  7. NATIONAL INSTITUTE OF NEUROLOGICAL DISORDERS AND STROKE [P30NS047101] Funding Source: NIH RePORTER
  8. Div Of Industrial Innovation & Partnersh [1561178] Funding Source: National Science Foundation

向作者/读者索取更多资源

The integration of three-dimensional micropatterning with microfluidics provides a unique opportunity to create perfusable tissue constructs in vitro. Herein, we have used this approach to create a tumor-on-a-chip with an endothelial barrier. Specifically, we photopatterned a mixture of endothelial cells and cancer spheroids within a gelatin methacrylate (GelMA) hydrogel inside a microfluidic device. The differential motility of endothelial and cancer cells in response to a controlled morphogen gradient across the cell-laden network drove the migration of endothelial cells to the periphery while maintaining the cancer cells within the interior of the hydrogel. The resultant endothelial cell layer forming cell-cell contact via VE-cadherin junctions was found to encompass the entire GelMA hydrogel structure. Furthermore, we have also examined the potential of such a tumor-on-a-chip system as a drug screening platform using doxorubicin, a model cancer drug.

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