期刊
LAB ON A CHIP
卷 16, 期 10, 页码 1821-1830出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c6lc00215c
关键词
-
类别
资金
- NSF Center for Advanced Design & Manufacturing of Integrated Microfluidics (CADMIM) [IIP-1362048, IIP-1362165]
- Micro/nano Fluidics Fundamentals Focus (MF3) Center at UC-Irvine
- Directorate For Engineering [1362165] Funding Source: National Science Foundation
- Div Of Industrial Innovation & Partnersh [1362048] Funding Source: National Science Foundation
Inertial microfluidics has been a highly active area of research in recent years for high-throughput focusing and sorting of synthetic and biological microparticles. However, existing inertial microfluidic devices always rely on microchannels with high-aspect-ratio geometries (channel width w < channel height h) and small cross-sections (w x h < 50 x 100 mu m(2)). Such deep and small structures increase fabrication difficulty and can limit manufacturing by large-scale and high-throughput production approaches such as roll-to-roll (R2R) hot embossing. In this work, we present a novel inertial microfluidic device using only a simple and low-aspect-ratio (LAR) straight microchannel (w > h) to achieve size-based sorting of microparticles and cells. The simple LAR geometry of the device enables successful high-throughput fabrication using R2R hot embossing. With optimized flow conditions and channel dimensions, we demonstrate continuous sorting of a mixture of 15 mu m and 10 mu m diameter microbeads with > 97% sorting efficiency using the low-cost and disposable R2R chip. We further demonstrate size-based sorting of bovine white blood cells, demonstrating the ability to process real cellular samples in our R2R chip. We envision that this R2R hot-embossed inertial microfluidic chip will serve as a powerful yet low-cost and disposable tool for size-based sorting of synthetic microparticles in industrial applications or cellular samples in cell biology research and clinical diagnostics.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据