期刊
ARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY
卷 51, 期 1, 页码 139-147出版社
TAYLOR & FRANCIS LTD
DOI: 10.1080/21691401.2023.2186887
关键词
Fluorescence; indocyanine green; imaging; liver cancer; nanoparticles
This study aimed to enhance the specific interactions between human hepatocellular carcinoma cell lines (HepG-2) by preparing and investigating the fluorescence emission properties of the Ag-Au-ICG complex. The complex was prepared via physical adsorption and evaluated for fluorescence spectra. The addition of an optimized dosage of Ag-Au-ICG to HepG-2 resulted in maximum fluorescence signal intensity and enhanced contrast fluorescence. The complex showed low cytotoxicity, making it a potential fluorescence enhancer for liver cancer imaging.
Indocyanine green (ICG) is effective for a variety of applications including liver tumour imaging and operates in the near-infrared window. Agents for near-infrared imaging are, however, still in clinical development. The present study aimed to prepare and investigate fluorescence emission properties of ICG in combination with Ag-Au in order to enhance their specific interactions with human hepatocellular carcinoma cell lines (HepG-2). The Ag-Au-ICG complex was prepared via physical adsorption, and hence evaluated for fluorescence spectra using a spectrophotometer. Ag-Au-ICG at an optimised dosage (Ag-Au:ICG = 0.0147:1 molar ratio) in Intralipid medium was added to HepG-2 to observe the maximum fluorescence signal intensity, which further enhanced HepG-2 contrast fluorescence. Ag-Au-ICG served as a fluorescence enhancer bound onto the liposome membrane, whilst free Ag, Au, and pure ICG induced low levels of cytotoxicity in HepG-2 and a normal human cell line. Thus, our findings provided new insights for the liver cancer imaging.
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