期刊
TOXINS
卷 15, 期 6, 页码 -出版社
MDPI
DOI: 10.3390/toxins15060362
关键词
atropine; scopolamine; tropane alkaloids; mu SPEed((R)); HPLC-MS/MS; teas/herbal teas; infusions; brewing conditions
This study analyzed 33 samples of tea and herbal tea infusions purchased in Spain and Portugal and found that 64% of the samples were contaminated with atropine and scopolamine. White and green teas were more contaminated compared to black and other herbal teas. Among the 21 contaminated samples, 15 had concentrations above the maximum limit set by the EU Commission Regulation (0.2 ng/mL) for liquid herbal infusions.
Atropine and scopolamine belong to the tropane alkaloid (TA) family of natural toxins. They can contaminate teas and herbal teas and appear in infusions. Therefore, this study focused on analyzing atropine and scopolamine in 33 samples of tea and herbal tea infusions purchased in Spain and Portugal to determine the presence of these compounds in infusions brewed at 97 degrees C for 5 min. A rapid microextraction technique ( mu SPEed((R))) followed by high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) was used to analyze the selected TAs. The results showed that 64% of the analyzed samples were contaminated by one or both toxins. White and green teas were generally more contaminated than black and other herbal teas. Of the 21 contaminated samples, 15 had concentrations above the maximum limit for liquid herbal infusions (0.2 ng/mL) set by Commission Regulation (EU) 2021/1408. In addition, the effects of heating conditions (time and temperature) on atropine and scopolamine standards and naturally contaminated samples of white, green, and black teas were evaluated. The results showed that at the concentrations studied (0.2 and 4 ng/mL), there was no degradation in the standard solutions. Brewing with boiling water (decoction) for 5 and 10 min allowed for higher extraction of TAs from dry tea to infusion water.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据