期刊
STEM CELLS TRANSLATIONAL MEDICINE
卷 12, 期 5, 页码 266-280出版社
OXFORD UNIV PRESS
DOI: 10.1093/stcltm/szad014
关键词
senescence; mesenchymal stromal cells; magnetic resonance relaxometry; live-cell assay
Detection of cellular senescence is important in analyzing the quality of cell therapy products. This study reports the development of a rapid live-cell assay that can detect senescent cells in heterogeneous MSC cultures. The assay uses microscale Magnetic Resonance Relaxometry to measure the T-2 relaxation time, which correlates strongly with senescence markers.
Detection of cellular senescence is important quality analytics of cell therapy products, including mesenchymal stromal cells (MSCs). However, its detection is critically limited by the lack of specific markers and the destructive assays used to read out these markers. Here, we establish a rapid, live-cell assay for detecting senescent cells in heterogeneous mesenchymal stromal cell (MSC) cultures. We report that the T-2 relaxation time measured by microscale Magnetic Resonance Relaxometry, which is related to intracellular iron accumulation, correlates strongly with senescence markers in MSC cultures under diverse conditions, including different passages and donors, size-sorted MSCs by inertial spiral microfluidic device, and drug-induced senescence. In addition, the live-cell and non-destructive method presented here has general applicability to other cells and tissues and can critically advance our understanding of cellular senescence.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据