Plasticity of Drosophila germ granules during germ cell development

Compartmentalization of RNAs and proteins into membraneless structures called granules is a ubiquitous mechanism for organizing and regulating cohorts of RNAs. Germ granules are ribonucleoprotein (RNP) assemblies required for germline development across the animal kingdom, but their regulatory roles in germ cells are not fully understood. We show that after germ cell specification, Drosophila germ granules enlarge through fusion and this growth is accompanied by a shift in function. Whereas germ granules initially protect their constituent mRNAs from degradation, they subsequently target a subset of these mRNAs for degradation while maintaining protection of others. This functional shift occurs through the recruitment of decapping and degradation factors to the germ granules, which is promoted by decapping activators and renders these structures P body-like. Disrupting either the mRNA protection or degradation function results in germ cell migration defects. Our findings reveal plasticity in germ granule function that allows them to be repurposed at different stages of development to ensure population of the gonad by germ cells. Additionally, these results reveal an unexpected level of functional complexity whereby constituent RNAs within the same granule type can be differentially regulated.

Automated summary

We discovered that Drosophila germ granules undergo fusion and a shift in function after germ cell specification. Initially, they protect constituent mRNAs from degradation, but later selectively target a subset of these mRNAs for degradation while still protecting others. This functional shift is mediated by the recruitment of decapping and degradation factors, turning the germ granules into P body-like structures. These findings demonstrate the plasticity of germ granule function and reveal a surprising level of complexity in the regulation of constituent RNAs.