期刊
GENOME MEDICINE
卷 15, 期 1, 页码 -出版社
BMC
DOI: 10.1186/s13073-023-01179-2
关键词
Diabetes; Human islet; Human pancreatic beta cell; Single-cell RNA sequencing; Single-nucleus RNA sequencing; Human islet graft
Single-cell RNA sequencing provides valuable insights into human islet cell types and their gene expression profiles, but it requires cell dissociation, limiting its in vivo application. In contrast, single-nucleus RNA sequencing is compatible with frozen samples, eliminates dissociation-induced stress responses, and provides enhanced information from intronic sequences for identifying pre-mRNA transcripts.
BackgroundSingle-cell RNA sequencing (scRNA-seq) provides valuable insights into human islet cell types and their corresponding stable gene expression profiles. However, this approach requires cell dissociation that complicates its utility in vivo. On the other hand, single-nucleus RNA sequencing (snRNA-seq) has compatibility with frozen samples, elimination of dissociation-induced transcriptional stress responses, and affords enhanced information from intronic sequences that can be leveraged to identify pre-mRNA transcripts.MethodsWe obtained nuclear preparations from fresh human islet cells and generated snRNA-seq datasets. We compared these datasets to scRNA-seq output obtained from human islet cells from the same donor. We employed snRNA-seq to obtain the transcriptomic profile of human islets engrafted in immunodeficient mice. In both analyses, we included the intronic reads in the snRNA-seq data with the GRCh38-2020-A library.ResultsFirst, snRNA-seq analysis shows that the top four differentially and selectively expressed genes in human islet endocrine cells in vitro and in vivo are not the canonical genes but a new set of non-canonical gene markers including ZNF385D, TRPM3, LRFN2, PLUT (beta-cells); PTPRT, FAP, PDK4, LOXL4 (alpha-cells); LRFN5, ADARB2, ERBB4, KCNT2 (delta-cells); and CACNA2D3, THSD7A, CNTNAP5, RBFOX3 (gamma-cells). Second, by integrating information from scRNA-seq and snRNA-seq of human islet cells, we distinguish three beta-cell sub-clusters: an INS pre-mRNA cluster (beta 3), an intermediate INS mRNA cluster (beta 2), and an INS mRNA-rich cluster (beta 1). These display distinct gene expression patterns representing different biological dynamic states both in vitro and in vivo. Interestingly, the INS mRNA-rich cluster (beta 1) becomes the predominant sub-cluster in vivo.ConclusionsIn summary, snRNA-seq and pre-mRNA analysis of human islet cells can accurately identify human islet cell populations, subpopulations, and their dynamic transcriptome profile in vivo.
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