期刊
G3-GENES GENOMES GENETICS
卷 13, 期 4, 页码 -出版社
OXFORD UNIV PRESS INC
DOI: 10.1093/g3journal/jkac339
关键词
hybrid genome assembly; Robertsonian polymorphism; chromosome fusion; domino damselfish; ONT; Hi-C Chicago; illumina shotgun; coral reef fish; Pomacentridae
In this study, the first-genome assembly of the three-spot damselfish was presented, revealing a heterozygous Robertsonian fusion karyotype and homologous chromosomes with the closely related clownfish. This assembly will be a valuable resource for population genomics and conservation research of damselfishes, as well as further studies on karyotypic diversity in this clade.
Damselfishes (Family: Pomacentridae) are a group of ecologically important, primarily coral reef fishes that include over 400 species. Damselfishes have been used as model organisms to study recruitment (anemonefishes), the effects of ocean acidification (spiny damselfish), population structure, and speciation (Dascyllus). The genus Dascyllus includes a group of small-bodied species, and a complex of relatively larger bodied species, the Dascyllus trimaculatus species complex that is comprised of several species including D. trimaculatus itself. The three-spot damselfish, D. trimaculatus, is a widespread and common coral reef fish species found across the tropical Indo-Pacific. Here, we present the first-genome assembly of this species. This assembly contains 910 Mb, 90% of the bases are in 24 chromosome-scale scaffolds, and the Benchmarking Universal Single-Copy Orthologs score of the assembly is 97.9%. Our findings confirm previous reports of a karyotype of 2n = 47 in D. trimaculatus in which one parent contributes 24 chromosomes and the other 23. We find evidence that this karyotype is the result of a heterozygous Robertsonian fusion. We also find that the D. trimaculatus chromosomes are each homologous with single chromosomes of the closely related clownfish species, Amphiprion percula. This assembly will be a valuable resource in the population genomics and conservation of Damselfishes, and continued studies of the karyotypic diversity in this clade.
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