Regional variation in cholinergic terminal activity determines the non-uniform occurrence of cortical slow waves during REM sleep in mice

Sleep consists of two basic stages: non-rapid eye movement (NREM) and rapid eye movement (REM) sleep. NREM sleep is characterized by slow high-amplitude cortical electroencephalogram (EEG) signals, while REM sleep is characterized by desynchronized cortical rhythms. Despite this, recent electrophysiological studies have suggested the presence of slow waves (SWs) in local cortical areas during REM sleep. Electro-physiological techniques, however, have been unable to resolve the regional structure of these activities because of relatively sparse sampling. Here, we map functional gradients in cortical activity during REM sleep using mesoscale imaging in mice and show local SW patterns occurring mainly in somatomotor and auditory cortical regions with minimum presence within the default mode network. The role of the cholinergic system in local desynchronization during REM sleep is also explored by calcium imaging of cholinergic activity within the cortex and analyzing structural data. We demonstrate weaker cholinergic projections and terminal activ-ity in regions exhibiting frequent SWs during REM sleep.

Automated summary

Sleep consists of NREM and REM stages. Recent studies have found slow wave activity in local cortical regions during REM sleep. Using mesoscale imaging in mice, we mapped cortical activity gradients during REM sleep and found that slow waves mainly occur in somatomotor and auditory regions, with fewer occurrences in the default mode network. Additionally, we explored the role of the cholinergic system in local desynchronization during REM sleep.