The cellular basis of mechanosensation in mammalian tongue

Mechanosensory neurons that innervate the tongue provide essential information to guide feeding, speech, and social grooming. We use in vivo calcium imaging of mouse trigeminal ganglion neurons to identify functional groups of mechanosensory neurons innervating the anterior tongue. These sensory neurons respond to thermal and mechanical stimulation. Analysis of neuronal activity patterns reveal that most mechanosensory trigeminal neurons are tuned to detect moving stimuli across the tongue. Us-ing an unbiased, multilayer hierarchical clustering approach to classify pressure-evoked activity based on temporal response dynamics, we identify five functional classes of mechanosensory neurons with distinct force-response relations and adaptation profiles. These populations are tuned to detect different features of touch. Molecular markers of functionally distinct clusters are identified by analyzing cluster represen-tation in genetically marked neuronal subsets. Collectively, these studies provide a platform for defining the contributions of functionally distinct mechanosensory neurons to oral behaviors crucial for survival in mammals.

Automated summary

Mechanosensory neurons innervating the tongue play a crucial role in feeding, speech, and social grooming. By using in vivo calcium imaging of mouse trigeminal ganglion neurons, different functional groups of these neurons can be identified. These neurons respond to thermal and mechanical stimuli and are tuned to detect moving stimuli across the tongue. The findings provide insights into the contributions of functionally distinct mechanosensory neurons in oral behaviors crucial for mammalian survival.