期刊
CHEMBIOCHEM
卷 17, 期 3, 页码 228-232出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/cbic.201500560
关键词
crowding; fluorescent probes; gene expression; hydrogel; microfluidics; spatial localization
资金
- European Research Council (ERC) [246812]
- VICI grant from the Netherlands Organization for Scientific Research (NWO)
- Dutch Ministry of Education, Culture and Science [024.001.035]
- European Research Council (ERC) [246812] Funding Source: European Research Council (ERC)
In highly crowded and viscous intracellular environments, the kinetics of complex enzymatic reactions are determined by both reaction and diffusion rates. However in vitro studies on transcription and translation often fail to take into account the density of the prokaryotic cytoplasm. Here we mimic the cellular environment by using a porous hydrogel matrix, to study the effects of macromolecular crowding on gene expression. We found that within microgels gene expression is localized, transcription is enhanced up to fivefold, and translation is enhanced up to fourfold. Our results highlight the need to consider the role of the physical environment on complex biochemical reactions, in this case macromolecular crowding, nanoscale spatial organization, and confinement.
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