New Aequorea Fluorescent Proteins for Cell-Free Bioengineering

Recently, a new subset of fluorescent proteins has been identified from the Aequorea species of jellyfish. These fluorescent proteins were characterized in vivo; however, there has not been validation of these proteins within cell-free systems. Cell-free systems and technology development is a rapidly expanding field, encompassing foundational research, synthetic cells, bioengineering, biomanufacturing, and drug development. Cell-free systems rely heavily on fluorescent proteins as reporters. Here we characterize and validate this new set of Aequorea proteins for use in a variety of cell-free and synthetic cell expression platforms.

Automated summary

Recently, a new subset of fluorescent proteins from Aequorea jellyfish has been discovered. These proteins have been characterized in vivo but not validated in cell-free systems. Cell-free systems are crucial in various fields including foundational research, synthetic cells, bioengineering, biomanufacturing, and drug development, and they heavily rely on fluorescent proteins as reporters. In this study, we characterize and validate this new set of Aequorea proteins for their use in cell-free and synthetic cell expression platforms.