期刊
STEM CELL RESEARCH & THERAPY
卷 14, 期 1, 页码 -出版社
BMC
DOI: 10.1186/s13287-023-03304-9
关键词
Inner ear regeneration; Stem cell transplantation; Xenorejection; Gene engineering; Hypoimmunogenic derivatives
Stem cell replacement shows potential for treating sensorineural hearing loss (SNHL). Immune evasion strategies can improve the survival and residence time of transplanted stem cells in the cochlea, leading to better outcomes.
Stem cell replacement holds the potential for sensorineural hearing loss (SNHL) treatment. However, its translation into clinical practice requires strategies for improving stem cell survival following intracochlear transplantation. Considering recent findings showing that the inner ear contains a resident population of immune cells, we hypothesized that immune evasion would improve the survival and residence time of transplanted stem cells in the cochlea, potentially leading to better outcomes. To test this, we leveraged genetic engineering techniques to develop hypoimmunogenic human-induced pluripotent stem cells (hi-iPSC), which lack human leukocyte antigen expression. We found that gene editing does not affect the biological properties of hi-iPSCs, including their capacity to differentiate into otic neural progenitors (ONPs). Compared to wild-type ONPs, more hypoimmunogenic ONPs (derived from hi-iPSCs) were found in the inner ear of immunocompetent mice ten days following cochlear xenotransplantation. This approach may open a new avenue for experimental and clinical SNHL treatments.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据