A new extremophile ostracod crustacean from the Movile Cave sulfidic chemoautotrophic ecosystem in Romania

Sulfidic cave ecosystems are remarkable evolutionary hotspots that have witnessed adaptive radiation of their fauna represented by extremophile species having particular traits. Ostracods, a very old group of crustaceans, exhibit specific morphological and ecophysiological features that enable them to thrive in groundwater sulfidic environments. Herein, we report a peculiar new ostracod species Pseudocandonamovilaensis sp. nov. thriving in the chemoautotrophic sulfidic groundwater ecosystem of Movile Cave (Romania). The new species displays a set of homoplastic features specific for unrelated stygobitic species, e.g., triangular carapace in lateral view with reduced postero-dorsal part and simplification of limb chaetotaxy (i.e., loss of some claws and reduction of secondary male sex characteristics), driven by a convergent or parallel evolution during or after colonization of the groundwater realm. P.movilaensis sp. nov. thrives exclusively in sulfidic meso-thermal waters (21 degrees C) with high concentrations of sulphides, methane, and ammonium. Based on the geometric morphometrics-based study of the carapace shape and molecular phylogenetic analyses based on the COI marker (mtDNA), we discuss the phylogenetic relationship and evolutionary implication for the new species to thrive in groundwater sulfidic groundwater environments.

Automated summary

We report a peculiar new ostracod species, Pseudocandonamovilaensis sp. nov., thriving in the chemoautotrophic sulfidic groundwater ecosystem of Movile Cave in Romania. The new species has homoplastic features, such as a triangular carapace in lateral view with a reduced postero-dorsal part and simplification of limb chaetotaxy, driven by convergent or parallel evolution during or after colonization of the groundwater realm. P. movilaensis sp. nov. exclusively thrives in sulfidic meso-thermal waters with high concentrations of sulphides, methane, and ammonium. Geometric morphometrics-based study of the carapace shape and molecular phylogenetic analyses based on the COI marker (mtDNA) were conducted to discuss the phylogenetic relationship and evolutionary implication for the new species to thrive in groundwater sulfidic environments.