4.7 Article

Application of self-organizing maps to AFM-based viscoelastic characterization of breast cancer cell mechanics

期刊

SCIENTIFIC REPORTS
卷 13, 期 1, 页码 -

出版社

NATURE PORTFOLIO
DOI: 10.1038/s41598-023-30156-3

关键词

-

向作者/读者索取更多资源

Cell mechanical properties can be used as label free markers for disease diagnosis, such as cancer. Atomic Force Microscopy (AFM) is commonly used to study cell mechanics, but requires skilled users, physical modeling, and data interpretation expertise. Recently, machine learning and artificial neural network techniques have been used to automatically classify AFM datasets. We propose the use of self-organizing maps (SOMs) as unsupervised artificial neural networks to analyze mechanical measurements on breast cancer cells treated with different substances that affect estrogen receptor signaling.
Cell mechanical properties have been proposed as label free markers for diagnostic purposes in diseases such as cancer. Cancer cells show altered mechanical phenotypes compared to their healthy counterparts. Atomic Force Microscopy (AFM) is a widely utilized tool to study cell mechanics. These measurements often need skilful users, physical modelling of mechanical properties and expertise in data interpretation. Together with the need to perform many measurements for statistical significance and to probe wide enough areas in tissue structures, the application of machine learning and artificial neural network techniques to automatically classify AFM datasets has received interest recently. We propose the use of self-organizing maps (SOMs) as unsupervised artificial neural network applied to mechanical measurements performed via AFM on epithelial breast cancer cells treated with different substances that affect estrogen receptor signalling. We show changes in mechanical properties due to treatments, as estrogen softened the cells, while resveratrol led to an increase in cell stiffness and viscosity. These data were then used as input for SOMs. Our approach was able to distinguish between estrogen treated, control and resveratrol treated cells in an unsupervised manner. In addition, the maps enabled investigation of the relationship of the input variables.

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.7
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

暂无数据
暂无数据