Rapid and efficient ambient temperature X-ray crystal structure determination at Turkish Light Source

High-resolution biomacromolecular structure determination is essential to better understand protein function and dynamics. Serial crystallography is an emerging structural biology technique which has fundamental limitations due to either sample volume requirements or immediate access to the competitive X-ray beamtime. Obtaining a high volume of well-diffracting, sufficient-size crystals while mitigating radiation damage remains a critical bottleneck of serial crystallography. As an alternative, we introduce the plate-reader module adapted for using a 72-well Terasaki plate for biomacromolecule structure determination at a convenience of a home X-ray source. We also present the first ambient temperature lysozyme structure determined at the Turkish light source (Turkish DeLight). The complete dataset was collected in 18.5 min with resolution extending to 2.39 angstrom and 100% completeness. Combined with our previous cryogenic structure (PDB ID: 7Y6A), the ambient temperature structure provides invaluable information about the structural dynamics of the lysozyme. Turkish DeLight provides robust and rapid ambient temperature biomacromolecular structure determination with limited radiation damage.

Automated summary

High-resolution biomacromolecular structure determination is crucial for understanding protein function and dynamics. Serial crystallography has limitations in terms of sample volume requirements and access to X-ray beamtime. To overcome these limitations, a plate-reader module adapted for a 72-well Terasaki plate is introduced for biomacromolecule structure determination using a home X-ray source. The first ambient temperature lysozyme structure determined at the Turkish light source (Turkish DeLight) provides valuable information about the structural dynamics of the lysozyme. Turkish DeLight offers robust and rapid ambient temperature biomacromolecular structure determination with minimal radiation damage.