期刊
NUTRIENTS
卷 15, 期 8, 页码 -出版社
MDPI
DOI: 10.3390/nu15081996
关键词
rotavirus; infection; 2-fucosyllactose; oligosaccharide
This study investigated the changes in intestinal gene expression after rotavirus infection and the effect of 2'-FL. The results showed that rotavirus infection upregulated host antiviral genes and downregulated genes involved in absorptive processes and intestinal maturation. Supplementation of 2'-FL reduced diarrhea symptoms caused by rotavirus infection, but had a similar effect on gene expression as in the control-infected group.
Viral infections are described as modifying host gene expression; however, there is limited insight regarding rotavirus (RV) infections. This study aimed to assess the changes in intestinal gene expression after RV infection in a preclinical model, and the effect of 2-fucosyllactose (2 '-FL) on this process. From days 2 to 8 of life, rats were supplemented with the dietary oligosaccharide 2 '-FL or vehicle. In addition, an RV was inoculated on day 5 to nonsupplemented animals (RV group) and to 2 '-FL-fed animals (RV+2 '-FL group). Incidence and severity of diarrhea were established. A portion from the middle part of the small intestine was excised for gene expression analysis by microarray kit and qPCR. In nonsupplemented animals, RV-induced diarrhea upregulated host antiviral genes (e.g., Oas1a, Irf7, Ifi44, Isg15) and downregulated several genes involved in absorptive processes and intestinal maturation (e.g., Onecut2, and Ccl19). The 2 '-FL-supplemented and infected animals had less diarrhea; however, their gene expression was affected in a similar way as the control-infected animals, with the exception of some immunity/maturation markers that were differentially expressed (e.g., Ccl12 and Afp). Overall, assessing the expression of these key genes may be useful in the evaluation of the efficacy of nutritional interventions or treatments for RV infection.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据