期刊
NUTRIENTS
卷 15, 期 7, 页码 -出版社
MDPI
DOI: 10.3390/nu15071730
关键词
celiac disease; ELISA; gluten-free diet; gluten immunogenic peptides; urine
A gluten-free diet is the only treatment for celiac disease, but adherence can be challenging due to the presence of gluten in many foods. This study proposes the development of an automatable ELISA to monitor adherence to the diet by quantifying urine gluten immunogenic peptides. The assay was validated and found to be stable, and it showed that after 12 months on the diet, only 27% of patients had detectable u-GIP compared to 100% at diagnosis. Therefore, this highly sensitive immunoassay could be used in clinical laboratories to analyze u-GIP from a large number of samples.
A gluten-free diet (GFD) is currently the only treatment available for patients with celiac disease (CD). However, adherence to a GFD can be challenging because gluten is present in many foods. A lifelong follow-up of patients with CD must be performed to promote adherence to a GFD and to identify the appearance of symptoms and the associated diseases. Therefore, the development of tools to analyze gluten exposure in these patients is important. This study proposes the development of the first automatable ELISA to monitor adherence to a GFD through the quantification of urine gluten immunogenic peptides (u-GIP). Seven healthy volunteers without suspicion of CD and 23 patients with CD were monitored as part of this study to optimize, validate, and apply this assay. Non-interference was found in the urine matrix, and the recovery percentage for spiked samples was 81-101%. The u-GIP was stable for up to 16 days when the samples were stored at different temperatures. Overall, 100% of the patients had detectable u-GIP at diagnosis (range of 0.39-2.14 ng GIP/mL), which reduced to 27% after 12 months on a GFD. Therefore, this highly sensitive immunoassay would allow the analysis of u-GIP from a large battery of samples in clinical laboratories of specialized healthcare centers.
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