期刊
CHEMBIOCHEM
卷 16, 期 12, 页码 1782-1790出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/cbic.201500247
关键词
biocontrol; natural products; secondary metabolism; triazine
资金
- USDA National Institute of Food and Agriculture through Agriculture and Food Research Initiative Competitive [2006-35319-17427, 2011-67019-30192]
- Economic Co-operation and Development
- College of Pharmacy, Oregon State University
- Robert A. Welch Foundation [A-0034]
- ARS [813314, ARS-0423042] Funding Source: Federal RePORTER
Pseudomonas spp. are prolific producers of natural products from many structural classes. Here we show that the soil bacterium Pseudomonas protegens Pf-5 is capable of producing trace levels of the triazine natural product toxoflavin (1) under microaerobic conditions. We evaluated toxoflavin production by derivatives of Pf-5 with deletions in specific biosynthesis genes, which led us to propose a revised biosynthetic pathway for toxoflavin that shares the first two steps with riboflavin biosynthesis. We also report that toxM, which is not present in the well-characterized cluster of Burkholderia glumae, encodes a monooxygenase that degrades toxoflavin. The toxoflavin degradation product of ToxM is identical to that of TflA, the toxoflavin lyase from Paenibacillus polymyxa. Toxoflavin production by P. protegens causes inhibition of several plant-pathogenic bacteria, and introduction of toxM into the toxoflavin-sensitive strain Pseudomonas syringae DC3000 results in resistance to toxoflavin.
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