期刊
DRUG DESIGN DEVELOPMENT AND THERAPY
卷 17, 期 -, 页码 389-402出版社
DOVE MEDICAL PRESS LTD
DOI: 10.2147/DDDT.S389764
关键词
gastric cancer; autophagy; AAMDC; solanine
This study investigated the effect of solanine on gastric cancer. The results showed that solanine suppressed gastric tumorigenesis and inhibited proliferation, invasion, and migration of gastric cancer cells. It also induced cell cycle arrest and apoptosis. The growth-limiting activities of solanine were attributed to the deactivation of the AAMDC-regulated autophagy pathway.
Purpose: Solanine is the main component of the plant Solanum, which has been shown to provide growth-limiting activities in a variety of human cancers. However, little is known about its function in gastric cancer (GC). Methods: We investigated the effect of solanine on GC in vivo and in vitro. The inhibition rate of solanine on the tumor was observed by constructing a subcutaneous tumor in nude mice. Morphological changes were analyzed with H&E staining. The expression of ATF4 was detected by IF analysis. MTT assays, EdU staining, and colony formation assays were used to detect the inhibition rate of solanine on GC cells. Matrigel transwells were used to detect the invasion of GC cells. Cell migration was measured using the wound healing assay. The flow cytometric analysis was used to monitor changes in the cell cycle and cell apoptosis. Western blotting was used to detect major proteins in cells and tumors. Results: Solanine suppressed gastric tumorigenesis. Solanine also inhibited the proliferation, invasion and mitigation of GC cells, and induced cell cycle arrest and apoptosis in vitro. Moreover, the growth-limiting activities of solanine in gastric cancer were related to the suppression of the AAMDC/MYC/ATF4/Sesn2 pathway-mediated autophagy. Overexpression of AAMDC reversed the inhibitory effect of solanine on autophagy and gastric cancer. Conclusion: In summary, our findings indicate that solanine confers growth-limiting activities by deactivating the AAMDC-regulated autophagy in gastric cancer.
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