Label-free intratissue activity imaging of alveolar organoids with dynamic optical coherence tomography

An organoid is a three-dimensional (3D) in vitro cell culture emulating human organs. We applied 3D dynamic optical coherence tomography (DOCT) to visualize the intratissue and intracellular activities of human induced pluripotent stem cells (hiPSCs)-derived alveolar organoids in normal and fibrosis models. 3D DOCT data were acquired with an 840-nm spectral domain optical coherence tomography with axial and lateral resolutions of 3.8 mu m (in tissue) and 4.9 mu m, respectively. The DOCT images were obtained by the logarithmic-intensity-variance (LIV) algorithm, which is sensitive to the signal fluctuation magnitude. The LIV images revealed cystic structures surrounded by high-LIV borders and mesh-like structures with low LIV. The former may be alveoli with a highly dynamics epithelium, while the latter may be fibroblasts. The LIV images also demonstrated the abnormal repair of the alveolar epithelium. Published by Optica Publishing Group under the terms of the Creative Commons Attribution 4.0 License. Further distribution of this work must maintain attribution to the author(s) and the published article's title, journal citation, and DOI.

Automated summary

We utilized 3D dynamic optical coherence tomography (DOCT) to observe the intratissue and intracellular activities of hiPSCs-derived alveolar organoids in normal and fibrosis models. The DOCT images revealed cystic structures surrounded by high-intensity borders, potentially representing alveoli with highly dynamic epithelium, as well as mesh-like structures, possibly fibroblasts. Furthermore, the images displayed abnormal repair of the alveolar epithelium.