Trumpet is an operating system for simple and robust cell-free biocomputing

Biological computation is becoming a viable and fast-growing alternative to traditional electronic computing. Here we present a biocomputing technology called Trumpet: Transcriptional RNA Universal Multi-Purpose GatE PlaTform. Trumpet combines the simplicity and robustness of the simplest in vitro biocomputing methods, adding signal amplification and programmability, while avoiding common shortcomings of live cell-based biocomputing solutions. We have demonstrated the use of Trumpet to build all universal Boolean logic gates. We have also built a web-based platform for designing Trumpet gates and created a primitive processor by networking several gates as a proof-of-principle for future development. The Trumpet offers a change of paradigm in biocomputing, providing an efficient and easily programmable biological logic gate operating system. Biological computation is becoming a viable and fast-growing alternative to traditional electronic computing. Here the authors present Trumpet, which uses DNA and enzymes to build logic gate circuits with amplified fluorescent readout.

Automated summary

Trumpet is a biocomputing technology that builds complex logic gate circuits using DNA and enzymes, with amplified fluorescent readout. It combines the simplicity and robustness of in vitro biocomputing methods, with signal amplification and programmability, avoiding common shortcomings of live cell-based biocomputing solutions.