Stimulation of lactic acid production and Lactobacillus plantarum growth in the coculture with Bacillus subtilis using jackfruit seed starch

Despite the exploration towards the environmentally friendly manufacturing process, two main challenges remain for lactic acid synthesis by microbial fermentation: resource cost and production efficiency. To alleviate environmental issues, lower raw material costs, and increase lactic acid manufacturing efficiency, this work aimed to evaluate characteristics of jackfruit seed starch (JFSS) extracted from fruit waste and explore the efficiency of lactic acid generation by co-fermentation of L. plantarum and B. subtilis in JFSS. JFSS consisted of a high amount of resistant starch and amylose, which was thermostable and displayed type A x-ray crystallization. Co-fermentation using JFSS as a substrate stimulated the growth of L. plantarum and enhanced lactic acid production over monocultures of L. plantarum and B. subtilis with insignificant by-product output. This study was valuable for improving lactic acid production efficiency and exploring the interaction between L. plantarum and B. subtilis.

Automated summary

Despite the challenges of resource cost and production efficiency, this study successfully used jackfruit seed starch (JFSS) to enhance lactic acid production efficiency through co-fermentation of L. plantarum and B. subtilis. The JFSS contained high amounts of resistant starch and amylose, and the co-fermentation stimulated the growth of L. plantarum without significant by-product output. This research contributes to improving lactic acid production efficiency and exploring the interaction between L. plantarum and B. subtilis.