Anatomic and neurochemical analysis of the palpal olfactory system in the red flour beetle Tribolium castaneum, HERBST

The paired antennal lobes were long considered the sole primary processing centers of the olfactory pathway in holometabolous insects receiving input from the olfactory sensory neurons of the antennae and mouthparts. In hemimetabolous insects, however, olfactory cues of the antennae and palps are processed separately. For the holometabolous red flour beetle Tribolium castaneum, we could show that primary processing of the palpal and antennal olfactory input also occurs separately and at distinct neuronal centers. While the antennal olfactory sensory neurons project into the antennal lobes, those of the palps project into the paired glomerular lobes and the unpaired gnathal olfactory center. Here we provide an extended analysis of the palpal olfactory pathway by combining scanning electron micrographs with confocal imaging of immunohistochemical staining and reporter expression identifying chemosensory and odorant receptor-expressing neurons in the palpal sensilla. In addition, we extended the anatomical characterization of the gnathal olfactory center by 3D reconstructions and investigated the distribution of several neuromediators. The similarities in the neuromediator repertoire between antennal lobes, glomerular lobes, and gnathal olfactory center underline the role of the latter two as additional primary olfactory processing centers.

Automated summary

Previously, it was believed that the paired antennal lobes were the only primary processing centers for olfactory input in holometabolous insects. However, we have discovered that in the red flour beetle Tribolium castaneum, olfactory input from the palps is also processed separately at distinct neuronal centers. The antennal olfactory sensory neurons project into the antennal lobes, while the palps project into the paired glomerular lobes and the unpaired gnathal olfactory center.