期刊
WORLD JOURNAL OF PEDIATRICS
卷 19, 期 10, 页码 1009-1016出版社
ZHEJIANG UNIV PRESS
DOI: 10.1007/s12519-023-00697-8
关键词
Divergent regions; Genotype-specific antibody; Human bocavirus 1 and 2; Major capsid protein VP3
类别
Through studying the divergent regions (DRs) on the major capsid protein VP3 of HBoV1 and HBoV2, it was found that DR2 had genotype specificity, reacting only with HBoV1. This study is important for accurate detection of HBoV1 and HBoV2 infections.
BackgroundPrevious serological studies of human bocavirus (HBoV) 1 could not exclude cross-reactivity with the other three HBoVs, particularly HBoV2.MethodsTo search for genotype-specific antibodies against HBoV1 and HBoV2, the divergent regions (DRs) located on the major capsid protein VP3 were defined through viral amino acid alignment and structure prediction. DR-deduced peptides were used as antigens to harvest corresponding anti-DR rabbit sera. To determine their genotype specificities for HBoV1 and HBoV2, these sera samples were used as antibodies against the antigens VP3 of HBoV1 and HBoV2 (expressed in Escherichia coli) in western blotting (WB), enzyme-linked immunosorbent assay (ELISA), and bio-layer interferometry (BLI) assays. Subsequently, the antibodies were evaluated with clinical specimens from pediatric patients with acute respiratory tract infection by indirect immunofluorescence assay (IFA).ResultsThere were four DRs (DR1-4) located on VP3 with different secondary and tertiary structures between HBoV1 and HBoV2. Regarding the reactivity with VP3 of HBoV1 or HBoV2 in WB and ELISA, high intra-genotype cross-reactivity of anti-HBoV1 or HBoV2 DR1, DR3, and DR4, but not anti-DR2, was observed. Genotype-specific binding capacity of anti-DR2 sera was confirmed by BLI and IFA, in which only anti-HBoV1 DR2 antibody reacted with HBoV1-positive respiratory specimens.ConclusionAntibodies against DR2, located on VP3 of HBoV1 or HBoV2, were genotype specific for HBoV1 and HBoV2, respectively.
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