期刊
VIROLOGY JOURNAL
卷 20, 期 1, 页码 -出版社
BMC
DOI: 10.1186/s12985-023-02068-1
关键词
SARS-CoV-2; Influenza a virus; Nipah virus; Enveloped virus; Viral entry; Membrane fusion; Syncytium; HTS; High-throughput screening
类别
In this study, a Bimolecular Multicellular Complementation (BiMuC) assay was developed to safely and efficiently monitor SARS-CoV-2 S-mediated membrane fusion without the need for microscopy-based equipment. Using BiMuC, a library of approved drugs was screened and compounds that enhance S protein-mediated cell-cell membrane fusion were identified, such as ethynylestradiol which promotes the growth of SARS-CoV-2 and Influenza A virus in vitro. These findings demonstrate the potential of BiMuC for identifying small molecules that modulate the life cycle of enveloped viruses, including SARS-CoV-2.
Several approaches have been developed to analyze the entry of highly pathogenic viruses. In this study, we report the implementation of a Bimolecular Multicellular Complementation (BiMuC) assay to safely and efficiently monitor SARS-CoV-2 S-mediated membrane fusion without the need for microscopy-based equipment. Using BiMuC, we screened a library of approved drugs and identified compounds that enhance S protein-mediated cell-cell membrane fusion. Among them, ethynylestradiol promotes the growth of SARS-CoV-2 and Influenza A virus in vitro. Our findings demonstrate the potential of BiMuC for identifying small molecules that modulate the life cycle of enveloped viruses, including SARS-CoV-2.
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