期刊
VETERINARY MICROBIOLOGY
卷 280, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.vetmic.2023.109703
关键词
Pseudorabies virus; Complete genome sequencing; Genomic recombination; Gene deletion; Virulence
In this study, two field strains of pseudorabies virus (PRV) were isolated and characterized. The GXLB-2015 strain showed genetic recombination between a foreign PRV strain and Chinese PRV variants, while the GXGG-2016 strain had a natural deletion in the thymidine kinase (TK) gene. Pathogenicity analysis revealed that GXLB-2015 strain was highly pathogenic to mice, while the GXGG-2016 strain was not pathogenic. These findings contribute to our understanding of PRV evolution in China and have implications for the control of PRV infection in pig farms.
Pseudorabies virus (PRV), the causative agent of Aujeszky's disease, has gained increased attention in China in recent years due to outbreaks of emergent pseudorabies. However, there is limited information about the evo-lution and pathogenicity of emergent PRV field strains in China. In this study, two PRV field strains were isolated from an intensive pig farm with suspected PRV infection. These were named the GXLB-2015 and GXGG-2016 strains and their growth characteristics together with their genome sequences and pathogenicity were deter-mined. Nucleotide homology and phylogenetic analysis revealed the GXLB-2015 stain was relatively close to the foreign PRV isolated strains with respect to the whole genome sequence. However, it formed an independent branch between the foreign PRV isolates and the previous PRV variants isolated in China. Further recombination and genetic evolution analysis showed that the GXLB-2015 strain was a natural recombinant between the Bartha strain and PRV variants. The GXGG-2016 strain was highly homologous with the Chinese classical strains, but it has a natural deletion of 69 aa in the thymidine kinase (TK) gene. Pathogenicity analysis showed that, the GXLB-2015 strain had the strongest pathogenicity to mice with an LD50 of 103.5, while the GXGG-2016 strain with the TK gene deletion was not pathogenic to mice. Taken together, our data provide direct evidence for the genomic recombination and natural TK gene deletion of PRVs, which may provide a reference for a better understanding of PRV evolution in China and contribute to the clinical control of PRV infection in pig farms.
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