期刊
JOURNAL OF ZHEJIANG UNIVERSITY-SCIENCE B
卷 17, 期 6, 页码 413-424出版社
ZHEJIANG UNIV
DOI: 10.1631/jzus.B1500261
关键词
Cell-cycle arrest; Cell injury; Heat stress; IEC-6 cell line; Magnolol
类别
资金
- National Natural Science Foundation of China [31272478]
- National Twelve-Five Technological Supported Plan of China [2013BAD10B04]
- Ministry of Agriculture, Public Service Sectors Agriculture Research Projects [201403051-07]
- Importation and Development of High-Caliber Talents Project of Beijing Municipal Institutions, China [CITTCD20130324]
Heat stress (HS) is an important environmental stressor that adversely influences livestock during the summer. The aim of this study was to investigate whether magnolol protects against HS-induced intestinal epithelial cell injury. An intestinal epithelial cell line (IEC-6) was subjected to HS at 42 A degrees C, with and without magnolol pretreatment. Cell injury was detected by monitoring lactate dehydrogenase (LDH) release. MTS (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium) assay was used to assess cell proliferation and viability, including identifying effective concentrations of magnolol. Flow cytometry confirmed G1-phase cell-cycle arrest and its alleviation by magnolol. Active DNA synthesis was measured by incorporation of nucleic acid 5-ethynyl-2'-deoxyuridine (EdU). G1-phase cell-cycle-related gene expression was assessed by real-time reverse transcription polymerase chain reaction (RT-PCR) and levels of G1-phase-related proteins by Western blotting. HS induced IEC-6 cell injury and decreased cell viability, as demonstrated by data from LDH and MTS assays, respectively. Based on a number of criteria, IEC-6 cells subjected to HS were arrested in the G1 phase of the cell cycle. Magnolol pretreatment decreased HS-induced cell injury through relief of this cell-cycle arrest. Magnolol pretreatment attenuates HS-induced injury in IEC-6 cells. Magnolol is potentially promising as a protective strategy for HS in livestock.
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