4.6 Review

Navigating the boundaries between metabolism and epigenetics in trypanosomes

相关参考文献

注意:仅列出部分参考文献,下载原文获取全部文献信息。
Article Microbiology

Genome-wide subcellular protein map for the flagellate parasite Trypanosoma brucei

Karen Billington et al.

Summary: Understanding the complex cell architecture and life cycle of Trypanosoma brucei, an important group of human, animal and plant unicellular parasites, is challenging due to a lack of knowledge about the functions of approximately 50% of its genome-encoded proteins. To address this, researchers developed TrypTag, a resource that maps the subcellular localization of 89% of the T. brucei proteome using fluorescence microscopy and cell lines expressing endogenously tagged proteins. TrypTag provides insights into function, defining lineage-specific organelle adaptations and allowing for the comprehensive analysis of eukaryotic flagella.

NATURE MICROBIOLOGY (2023)

Article Microbiology

H2B.V demarcates divergent strand-switch regions, some tDNA loci, and genome compartments in Trypanosoma cruzi and affects parasite differentiation and host cell invasion

Juliana Nunes Roson et al.

Summary: This study focused on the unique features of gene expression regulation in trypanosomatids. Through histone ChIP-seq analysis, it was found that H2B.V was enriched at critical genomic regions in T. cruzi and interacted with chromatin acetylation factors. Parasites with reduced H2B.V levels showed higher rates of differentiation and infectivity.

PLOS PATHOGENS (2022)

Article Biology

SWI/SNF senses carbon starvation with a pH-sensitive low-complexity sequence

J. Ignacio Gutierrez et al.

Summary: It is increasingly recognized that changes in intracellular pH serve as important biological signals. This study focuses on elucidating the molecular mechanisms of pH sensing. The researchers found that a nucleocytoplasmic pH oscillation is crucial for the transcriptional response to carbon starvation in Saccharomyces cerevisiae. They also discovered that the SWI/SNF chromatin remodeling complex plays a key role in this response. Specifically, a glutamine-rich low-complexity domain (QLC) in the SNF5 subunit of this complex, along with histidines within this domain, is essential for efficient transcriptional reprogramming. Additionally, the SNF5 QLC is responsible for the pH-dependent recruitment of SWI/SNF to an acidic transcription factor in a reconstituted nucleosome remodeling assay. Simulations revealed that the protonation of histidines in the SNF5 QLC causes conformational expansion, offering a potential biophysical mechanism for regulating these interactions. Overall, this study suggests that pH changes act as a second messenger for transcriptional reprogramming during carbon starvation, with the SNF5 QLC serving as a pH sensor.
Article Multidisciplinary Sciences

Enzymatic transfer of acetate on histones from lysine reservoir sites to lysine activating sites

Mariel Mendoza et al.

Summary: This study reveals the regulation of histone acetylation by nuclear acetyl-CoA pools and suggests a local transfer of acetate occurs during gene activation. The yeast ortholog of ACSS2, Acs2, is recruited to chromatin during quiescence exit and is preferentially associated with the most up-regulated genes, indicating the important role of acetyl group transfer in gene activation.

SCIENCE ADVANCES (2022)

Article Microbiology

Metabolic Alteration of Trypanosoma cruzi during Differentiation of Epimastigote to Trypomastigote Forms

Salvatore G. De-Simone et al.

Summary: This study investigated the energetic metabolism of T. cruzi during metacyclogenesis and provided insights into its differential metabolic modifications. It improved the understanding of T. cruzi metabolism by measuring enzymatic activities and organic acid concentrations.

PATHOGENS (2022)

Article Genetics & Heredity

Open chromatin analysis in Trypanosoma cruzi life forms highlights critical differences in genomic compartments and developmental regulation at tDNA loci

Alex Ranieri Jeronimo Lima et al.

Summary: This study investigated the impact of chromatin changes on the differentiation process of Trypanosoma cruzi, identifying differences in genome compartments and the regulation of tRNA loci. The results suggest a positive correlation between active chromatin and gene transcription levels, reflecting the unique gene expression regulation in trypanosomes.

EPIGENETICS & CHROMATIN (2022)

Article Biochemistry & Molecular Biology

Trypanosoma brucei histones are heavily modified with combinatorial post-translational modifications and mark Pol II transcription start regions with hyperacetylated H2A

Johannes P. Maree et al.

Summary: This study explores the complex epigenetic mechanisms employed by Trypanosoma brucei, a parasite diverged from the main eukaryotic lineage about 600 million years ago. The researchers found that core histones in T. brucei have extensive post-translational modifications (PTMs) and display intricate combinatorial PTM patterns. Hyperacetylated H2A histones were observed to be enriched at Pol II transcription start regions and displayed different genomic localizations based on their acetylation combinations, indicating distinct epigenetic functions. These findings provide valuable insights into the early processes employed by eukaryotic ancestors to regulate chromatin-mediated functions.

NUCLEIC ACIDS RESEARCH (2022)

Article Biology

The SPARC complex defines RNAPII promoters in Trypanosoma brucei

Desislava P. Staneva et al.

Summary: This study reveals the unique gene expression regulation mechanism in kinetoplastids, a highly divergent lineage of eukaryotes. The researchers identified a complex called SPARC, which consists of SET27 and CRD1, among other components, that is involved in establishing accurate promoter position and directionality.
Article Microbiology

Nucleosome landscape reflects phenotypic differences in Trypanosoma cruzi life forms

Alex R. J. Lima et al.

Summary: This study revealed differences in nucleosome organization at strategic genomic regions among replicative and nonreplicative T. cruzi forms. The nonreplicative forms have less dynamic nucleosomes, while dynamic nucleosomes are enriched at regulatory transcription initiation regions and multigenic family members associated with infective stage and virulence factors. Genes related to DNA replication, cytokinesis, transcription and translation regulation are mainly enriched on dynamic nucleosomes.

PLOS PATHOGENS (2021)

Article Microbiology

Spatial integration of transcription and splicing in a dedicated compartment sustains monogenic antigen expression in African trypanosomes

Joana Faria et al.

Summary: The study reveals a mechanism in Trypanosoma brucei where a single expressed antigen-coding gene interacts with a major messenger RNA splicing locus in a specific nuclear compartment, ensuring monogenic expression. Specific proteins VEX1 and VEX2 are associated with an antigen exclusion complex, playing a role in this process of antigen transcription and mRNA splicing. Depletion of VEX2 results in loss of monogenic antigen expression and increased interactions between previously silent antigen genes and the splicing locus.

NATURE MICROBIOLOGY (2021)

Article Parasitology

Carbohydrate metabolism in trypanosomatids: New insights revealing novel complexity, diversity and species-unique features

Paul A. M. Michels et al.

Summary: The carbohydrate metabolism of TriTryp parasites, including Trypanosoma brucei, Trypanosoma cruzi, and Leishmania spp., plays a central role in their biology, adapting to different nutrients throughout their complex life cycles. Differences in the expression and regulation of metabolic pathways among these parasites reflect their adaptation to various environments and hosts, indicating separate evolutionary tracks from a common ancestor.

EXPERIMENTAL PARASITOLOGY (2021)

Article Microbiology

Fatty acid oxidation participates in resistance to nutrient-depleted environments in the insect stages of Trypanosoma cruzi

Rodolpho Ornitz Oliveira Souza et al.

Summary: The study demonstrates that Trypanosoma cruzi undergoes continuous metabolic and cell cycle changes through gene epigenetic regulation in hosts, facilitating its survival in nutrient-poor environments.

PLOS PATHOGENS (2021)

Article Multidisciplinary Sciences

An updated map of Trypanosoma cruzi histone post-translational modifications

Rafael Fogaca de Almeida et al.

Summary: Histone post-translational modifications (hPTMs) are abundant and varied in Trypanosoma cruzi, indicating potential conserved epigenetic functions. The comprehensive map of hPTMs provided by this study can serve as a basis for further research on epigenetic mechanisms and the development of epigenetic drugs against trypanosomatids.

SCIENTIFIC DATA (2021)

Article Biochemistry & Molecular Biology

A systematic analysis of Trypanosoma brucei chromatin factors identifies novel protein interaction networks associated with sites of transcription initiation and termination

Desislava P. Staneva et al.

Summary: Histones form nucleosomes around which DNA is wrapped to create chromatin, and histone post-translational modifications (PTMs) play a role in regulating gene expression. A study on Trypanosoma brucei identified proteins involved in histone acetylation and methylation, showing associations with specific regions of transcription initiation and termination. Proteomic analyses revealed novel components in the chromatin regulatory network of T. brucei, indicating divergence from conventional eukaryotic norms.

GENOME RESEARCH (2021)

Review Cell Biology

Histone succinylation and its function on the nucleosome

Jiayi Liu et al.

Summary: Lysine succinylation is a newly identified histone post-translational modification that influences nucleosomal structure, chromatin dynamics, gene expression, and several diseases. Studies on lysine succinylation of histones are ongoing, highlighting its potential as a target for disease treatment in the future.

JOURNAL OF CELLULAR AND MOLECULAR MEDICINE (2021)

Article Biochemistry & Molecular Biology

Trimethylation of histone H3K76 by Dot1B enhances cell cycle progression after mitosis in Trypanosoma cruzi

Vinicius Santana Nunes et al.

BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH (2020)

Review Immunology

The Influence of Environmental Cues on the Development of Trypanosoma cruzi in Triatominae Vector

Raissa de Fatima Pimentel Melo et al.

FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY (2020)

Article Multidisciplinary Sciences

Distinct roles for H4 and H2A.Z acetylation in RNA transcription in African trypanosomes

Amelie J. Kraus et al.

NATURE COMMUNICATIONS (2020)

Review Endocrinology & Metabolism

Chromatin -remodeling links metabolic signaling to gene expression

Ashby J. Morrison

MOLECULAR METABOLISM (2020)

Review Biochemistry & Molecular Biology

Regulation of gene expression in trypanosomatids: living with polycistronic transcription

Christine Clayton

OPEN BIOLOGY (2019)

Article Biochemistry & Molecular Biology

L-Glutamine uptake is developmentally regulated and is involved in metacyclogenesis in Trypanosoma cruzi

Flavia S. Damasceno et al.

MOLECULAR AND BIOCHEMICAL PARASITOLOGY (2018)

Review Biochemistry & Molecular Biology

Spatiotemporal Control of Acetyl-CoA Metabolism in Chromatin Regulation

Sharanya Sivanand et al.

TRENDS IN BIOCHEMICAL SCIENCES (2018)

Review Endocrinology & Metabolism

Sink into the Epigenome: Histones as Repositories That Influence Cellular Metabolism

Cunqi Ye et al.

TRENDS IN ENDOCRINOLOGY AND METABOLISM (2018)

Article Genetics & Heredity

Expanding an expanded genome: long-read sequencing of Trypanosoma cruzi

Luisa Berna et al.

MICROBIAL GENOMICS (2018)

Article Biochemical Research Methods

Quantitative Proteomic Analysis of Replicative and Nonreplicative Forms Reveals Important Insights into Chromatin Biology of Trypanosoma cruzi

Teresa Cristina Leandro de Jesus et al.

MOLECULAR & CELLULAR PROTEOMICS (2017)

Article Biochemistry & Molecular Biology

Metabolomic profiling reveals a finely tuned, starvation-induced metabolic switch in Trypanosoma cruzi epimastigotes

Maria Julia Barison et al.

JOURNAL OF BIOLOGICAL CHEMISTRY (2017)

Article Biochemical Research Methods

Post-translational Modifications of Trypanosoma cruzi Canonical and Variant Histones

Gisele F. A. Picchi et al.

JOURNAL OF PROTEOME RESEARCH (2017)

Article Biochemistry & Molecular Biology

A Metabolic Function for Phospholipid and Histone Methylation

Cunqi Ye et al.

MOLECULAR CELL (2017)

Article Biochemistry & Molecular Biology

Nuclear Acetyl-CoA Production by ACLY Promotes Homologous Recombination

Sharanya Sivanand et al.

MOLECULAR CELL (2017)

Review Biochemistry & Molecular Biology

Metabolic control by sirtuins and other enzymes that sense NAD+, NADH, or their ratio

Kristin A. Anderson et al.

BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS (2017)

Article Biochemistry & Molecular Biology

GT-rich promoters can drive RNA pol II transcription and deposition of H2A.Z in African trypanosomes

Carolin Wedel et al.

EMBO JOURNAL (2017)

Review Biochemistry & Molecular Biology

Biogenesis, maintenance and dynamics of glycosomes in trypanosomatid parasites

Jurgen R. Haanstra et al.

BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH (2016)

Article Biochemical Research Methods

Chromatin Proteomics Reveals Variable Histone Modifications during the Life Cycle of Trypanosoma cruzi

Teresa Cristina Leandro de Jesus et al.

JOURNAL OF PROTEOME RESEARCH (2016)

Review Cell Biology

Metabolism and Epigenetics

Ryan Janke et al.

ANNUAL REVIEW OF CELL AND DEVELOPMENTAL BIOLOGY, VOL 31 (2015)

Article Biochemistry & Molecular Biology

Expression of non-acetylatable lysines 10 and 14 of histone H4 impairs transcription and replication in Trypanosoma cruzi

Thiago Cesar Prata Ramos et al.

MOLECULAR AND BIOCHEMICAL PARASITOLOGY (2015)

Review Biochemistry & Molecular Biology

Mitochondrial Proteins Moonlighting in the Nucleus

Richard M. Monaghan et al.

TRENDS IN BIOCHEMICAL SCIENCES (2015)

Article Multidisciplinary Sciences

Proline Dehydrogenase Regulates Redox State and Respiratory Metabolism in Trypanosoma cruzi

Lisvane Silva Paes et al.

PLOS ONE (2013)

Review Biochemistry & Molecular Biology

Evolutionary cell biology of chromosome segregation: insights from trypanosomes

Bungo Akiyoshi et al.

OPEN BIOLOGY (2013)

Article Biochemistry & Molecular Biology

Glucosylated Hydroxymethyluracil, DNA Base J, Prevents Transcriptional Readthrough in Leishmania

Henri G. A. M. van Luenen et al.

Article Biochemistry & Molecular Biology

JBP1 and JBP2 Proteins Are Fe2+/2-Oxoglutarate-dependent Dioxygenases Regulating Hydroxylation of Thymidine Residues in Trypanosome DNA

Laura J. Cliffe et al.

JOURNAL OF BIOLOGICAL CHEMISTRY (2012)

Review Cell Biology

Regulation of chromatin by histone modifications

Andrew J. Bannister et al.

CELL RESEARCH (2011)

Article Biophysics

A comparative assessment of mitochondrial function in epimastigotes and bloodstream trypomastigotes of Trypanosoma cruzi

Renata L. S. Goncalves et al.

JOURNAL OF BIOENERGETICS AND BIOMEMBRANES (2011)

Article Biochemistry & Molecular Biology

Functional characterization of NADP-dependent isocitrate dehydrogenase isozymes from Trypanosoma cruzi

Alejandro E. Leroux et al.

MOLECULAR AND BIOCHEMICAL PARASITOLOGY (2011)

Article Biochemistry & Molecular Biology

Epigenetic Regulation of Transcription and Virulence in Trypanosoma cruzi by O-Linked Thymine Glucosylation of DNA

Dilrukshi K. Ekanayake et al.

MOLECULAR AND CELLULAR BIOLOGY (2011)

Article Biochemistry & Molecular Biology

Histone H3 trimethylated at lysine 4 is enriched at probable transcription start sites in Trypanosoma brucei

Jessica R. Wright et al.

MOLECULAR AND BIOCHEMICAL PARASITOLOGY (2010)

Article Biochemistry & Molecular Biology

Histone deacetylases play distinct roles in telomeric VSG expression site silencing in African trypanosomes

Qiao-Ping Wang et al.

MOLECULAR MICROBIOLOGY (2010)

Article Biochemistry & Molecular Biology

Distinct acetylation of Trypanosoma cruzi histone H4 during cell cycle, parasite differentiation, and after DNA damage

Sheila Cristina Nardelli et al.

CHROMOSOMA (2009)

Article Biochemistry & Molecular Biology

Glucose uptake in the mammalian stages of Trypanosoma cruzi

Ariel M. Silber et al.

MOLECULAR AND BIOCHEMICAL PARASITOLOGY (2009)

Article Biochemistry & Molecular Biology

JBP1 and JBP2 are two distinct thymidine hydroxylases involved in J biosynthesis in genomic DNA of African trypanosomes

Laura J. Cliffe et al.

NUCLEIC ACIDS RESEARCH (2009)

Article Multidisciplinary Sciences

Actions of a Proline Analogue, L-Thiazolidine-4-Carboxylic Acid (T4C), on Trypanosoma cruzi

Anahi Magdaleno et al.

PLOS ONE (2009)

Article Multidisciplinary Sciences

Acetate produced in the mitochondrion is the essential precursor for lipid biosynthesis in procyclic trypanosomes

Loiec Riviere et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2009)

Article Multidisciplinary Sciences

ATP-Citrate Lyase Links Cellular Metabolism to Histone Acetylation

Kathryn E. Wellen et al.

SCIENCE (2009)

Article Multidisciplinary Sciences

Active transcription and ultrastructural changes during Trypanosoma cruzi metacyclogenesis

Ludmila R. P. Ferreira et al.

ANAIS DA ACADEMIA BRASILEIRA DE CIENCIAS (2008)

Article Biochemistry & Molecular Biology

Histone acetylation and methylation at sites initiating divergent polycistronic transcription in Trypanosoma cruzi

Patricia Respuela et al.

JOURNAL OF BIOLOGICAL CHEMISTRY (2008)

Article Biochemistry & Molecular Biology

Telomeric co-localization of the modified base J and contingency genes in the protozoan parasite Trypanosoma cruzi

Dilrukshi K. Ekanayake et al.

NUCLEIC ACIDS RESEARCH (2007)

Article Biochemistry & Molecular Biology

Characterization of a Trypanosoma cruzi acetyltransferase:: cellular location, activity and structure

Stephen Ochaya et al.

MOLECULAR AND BIOCHEMICAL PARASITOLOGY (2007)

Article Biochemistry & Molecular Biology

Post-translational modifications of Trypanosoma cruzi histone H4

Julia Pinheiro Chagas da Cunha et al.

MOLECULAR AND BIOCHEMICAL PARASITOLOGY (2006)

Review Biochemistry & Molecular Biology

Energy metabolism of trypanosomatids: Adaptation to available carbon sources

Frederic Bringaud et al.

MOLECULAR AND BIOCHEMICAL PARASITOLOGY (2006)

Article Biochemistry & Molecular Biology

Trypanosoma cruzi histone H1 is phosphorylated in a typical cyclin dependent kinase site accordingly to the cell cycle

JPC da Cunha et al.

MOLECULAR AND BIOCHEMICAL PARASITOLOGY (2005)

Article Biochemistry & Molecular Biology

Formation of linear inverted repeat amplicons following targeting of an essential gene in Leishmania

PA Genest et al.

NUCLEIC ACIDS RESEARCH (2005)

Article Biochemistry & Molecular Biology

Pyruvate phosphate dikinase and pyrophosphate metabolism in the glycosome of Trypanosoma cruzi epimastigotes

H Acosta et al.

COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY (2004)

Article Biochemistry & Molecular Biology

Transcription rate modulation through the Trypanosoma cruzi life cycle occurs in parallel with changes in nuclear organisation

MCQB Elias et al.

MOLECULAR AND BIOCHEMICAL PARASITOLOGY (2001)