State of the art in CRISPR/Cas system-based signal conversion and amplification applied in the field of food analysis

Article Chemistry, Analytical

Applying CRISPR/Cas system as a signal enhancer for DNAzyme-based lead ion detection

Yanju Chen et al.

Summary: A highly sensitive lead detection method combining DNAzyme and CRISPR system is proposed, which can detect low concentrations of lead ions and visualize the results with a portable 3D printing device, suitable for on-site detection.

ANALYTICA CHIMICA ACTA (2022)

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Article Biophysics

A rapid and ultrasensitive dual detection platform based on Cas12a for simultaneous detection of virulence and resistance genes of drug-resistant Salmonella

Xuran Fu et al.

Summary: This study developed a novel detection platform that can accurately and rapidly detect Salmonella and drug resistance genes with ultrahigh sensitivity. Cas12a-Ddp is not only suitable for rapid detection, but also applicable for detecting drug-resistant Salmonella in different liquid foods, with the potential for onsite application.

BIOSENSORS & BIOELECTRONICS (2022)

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Article Engineering, Environmental

A novel CRISPR/Cas14a system integrated with 2D porphyrin metal-organic framework for microcystin-LR determination through a homogeneous competitive reaction

Pian Wu et al.

Summary: A novel and versatile fluorescence sensor, Cas14-pMOFs fluorescence sensor, was developed for highly efficient detection of MC-LR by combining the CRISPR/Cas14a system with 2D-pMOFs. This sensor showed excellent performance and universality in detecting both nucleic acid and non-nucleic acid targets.

JOURNAL OF HAZARDOUS MATERIALS (2022)

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Article Chemistry, Analytical

CRISPR-Cas system meets DNA barcoding: Development of a universal nucleic acid test for food authentication

Yinhuan Wu et al.

Summary: The study introduces a new method for food authentication using the CRISPR-Cas system, which can detect DNA barcodes for specific molecular traceability of meat species. By combining the CRISPR/Cas12 system with the PCR method, this approach enhances the accuracy of identifying pork components.

SENSORS AND ACTUATORS B-CHEMICAL (2022)

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Article Food Science & Technology

Advances in optical-sensing strategies for the on-site detection of pesticides in agricultural foods

Reddicherla Umapathi et al.

Summary: Recent scientific and technological studies on optical sensing strategies for on-site detection of pesticide residues in agricultural foods have made novel advancements, including fluorescence sensors, hydrogels, chemiluminescence assay, enzyme-linked immunosorbent assays, fiber-optic biosensors, phosphorescence, lateral flow immunoassays, double-signal fluorescence strategies, glove-based sensors, and paper-based sensors. These advancements provide significant insights and future perspectives for the design and development of novel optical sensors with applications in various fields.

TRENDS IN FOOD SCIENCE & TECHNOLOGY (2022)

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Article Biochemical Research Methods

Label-Free Detection of Transgenic Crops Using an Isothermal Amplification Reporting CRISPR/Cas12 Assay

Xiaoying Zhu et al.

Summary: This study introduced a CRISPR/Cas system for detecting transgenic crops, allowing label-free and direct recognition of transgenes through isothermal amplification. The use of Cas12a and rolling circle amplification enhanced the sensitivity of the assay, enabling specific detection of transgenes. The method, termed isoCRISPR, can distinguish transgenic corn cultivars from nontransgenic ones, enriching the toolbox for transgenic crop identification.

ACS SYNTHETIC BIOLOGY (2022)

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Article Biophysics

A one-pot CRISPR/Cas13a-based contamination-free biosensor for low-cost and rapid nucleic acid diagnostics

Fei Hu et al.

Summary: A one-pot CRISPR/Cas13a-based visual biosensor was developed for rapid and low-cost nucleic acid detection. This method meets the need for rapid nucleic acid detection at home and on-site, and is suitable for resource-limited areas and epidemic outbreaks. The system has a limit of detection of 3 copy/μL and provides accurate results with shorter time and lower cost.

BIOSENSORS & BIOELECTRONICS (2022)

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Article Biophysics

CRISPR/Cas12a-Derived electrochemical aptasensor for ultrasensitive detection of COVID-19 nucleocapsid protein

Cong Han et al.

Summary: In this study, a CRISPR/Cas12a-derived electrochemical aptasensor for fast and ultrasensitive detection of COVID-19 nucleocapsid protein (Np) was developed. The sensor exhibited high selectivity to Np and could complete a test within 30 minutes. It showed great potential in early diagnosis of COVID-19, environmental surveillance, and food security.

BIOSENSORS & BIOELECTRONICS (2022)

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Article Chemistry, Applied

Inhibition of CRISPR-Cas12a trans-cleavage by lead (II)-induced G-quadruplex and its analytical application

Ying Yu et al.

Summary: In this study, the inhibition of CRISPR-Cas trans-cleavage by Pb2+-induced G-quadruplex was investigated for the detection of Pb2+ in tea beverage and milk. The results showed that Pb2+ competitively combines with G-quadruplex, resulting in its resistance to CRISPR-Cas trans-cleavage. The detection of Pb2+ was achieved through fluorescence resonance energy transfer.

FOOD CHEMISTRY (2022)

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Article Chemistry, Analytical

High-throughput microfluidic strategy based on RAA-CRISPR/Cas13a dual signal amplification for accurate identification of pathogenic Listeria

Xinran Xiang et al.

Summary: This study developed a one-step detection method (hMC-CRISPR) based on a high-throughput microfluidic chip for detecting pathogenic Listeria. The method combines recombinase aided amplification (RAA) and CRISPR technology, and achieves high specificity, sensitivity, and user-friendliness in detecting the pathogen in about 60 minutes.

SENSORS AND ACTUATORS B-CHEMICAL (2022)

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Article Chemistry, Analytical

Filtration-based LAMP-CRISPR/Cas12a system for the rapid, sensitive and visualized detection of Escherichia coli O157:H7

So-Young Lee et al.

Summary: In this study, a rapid, sensitive, and visualized method of detecting E. coli O157:H7 in fresh products was developed, which rectified the common false-negative results and had a high detection sensitivity.

TALANTA (2022)

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Article Food Science & Technology

CRISPR/Cas12a-based technology: A powerful tool for biosensing in food safety

Zefeng Mao et al.

Summary: The use of molecular detection in food safety has become urgent due to the detection of novel coronavirus events in the food cold chain. This review introduces a new molecular diagnostic technology based on CRISPR-Cas12a and summarizes its applications in various aspects of food safety. The biosensor analytical technology has great potential in qualitative and quantitative analysis of infectious pathogens.

TRENDS IN FOOD SCIENCE & TECHNOLOGY (2022)

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Article Chemistry, Multidisciplinary

Toward Mail-in-Sensors for SARS-CoV-2 Detection: Interfacing Gel Switch Resonators with Cell-Free Toehold Switches

Adam R. Carr et al.

Summary: The importance of widespread testing in controlling the spread of infectious diseases has been emphasized by the COVID-19 pandemic. This study demonstrates a testing platform using a screen-printed LC resonator as a transducer and a toehold switch coupled with cell-free expression as the biological selective recognition element. The platform shows good selectivity to the SARS-CoV-2 virus and has a low limit of detection.

ACS SENSORS (2022)

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Review Food Science & Technology

Cooperation and competition between CRISPR- and omics-based technologies in foodborne pathogens detection: a state of the art review

Yang Sun et al.

Summary: Foodborne pathogens are the main cause of food safety incidents, and rapid and accurate detection is crucial for human life and health. Food omics and CRISPR-based technologies have broad potential in detecting and editing foodborne pathogens, and combining big data with high sensitivity can develop more effective and convenient detection platforms to eliminate pathogens in food matrices.

CURRENT OPINION IN FOOD SCIENCE (2022)

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Article Biophysics

SERS-based CRISPR/Cas assay on microfluidic paper analytical devices for supersensitive detection of pathogenic bacteria in foods

Jianwen Zhuang et al.

Summary: In this study, a RPA-Cas12a-mu PAD was designed using CRISPR/Cas12a and SERS technology for highly sensitive bacterial detection. The concentration of S.typhi was determined by the aggregation level of SERS nanoprobes, enabling fast and accurate testing of food samples.

BIOSENSORS & BIOELECTRONICS (2022)

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Article Engineering, Environmental

CRISPR-Cas12a-mediated luminescence resonance energy transfer aptasensing platform for deoxynivalenol using gold nanoparticle-decorated Ti3C2Tx MXene as the enhanced quencher

Xianfeng Lin et al.

Summary: In this study, a sensitive and robust CRISPR-Cas12a-mediated aptasensor was developed to detect DON using labeled nanoparticles and enhanced quenchers. The sensor enables accurate analysis of DON and expands the detection range of CRISPR-Cas sensors for nonnucleic acid hazards in the environment and food.

JOURNAL OF HAZARDOUS MATERIALS (2022)

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Article Nanoscience & Nanotechnology

Nanozyme-catalysed CRISPR assay for preamplification-free detection of non-coding RNAs

Marta Broto et al.

Summary: This study combines CRISPR-Cas reaction with nanozyme-linked ELISA to achieve quantitative detection of Cas13-mediated RNA detection through catalytic metallic nanoparticles at room temperature (CrisprZyme). The study shows that CrisprZyme is adaptable to lateral flow reading and different Cas enzymes, and can detect various non-coding RNAs. The platform can be used to identify disease patients and monitor cellular differentiation. CrisprZyme can serve as a universally applicable signal catalyst for CRISPR-based diagnostics, expanding the target range of preamplification-free, quantitative detection.

NATURE NANOTECHNOLOGY (2022)

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Article Food Science & Technology

Monitoring and detection of antibiotic residues in animal derived foods: Solutions using aptamers

Yuan Liu et al.

Summary: Food safety, especially the issue of antibiotic residues in animal derived foods, has been a major concern for public health. Conventional techniques for monitoring and detecting antibiotics have limitations, but the advent of aptamers has provided a new approach. Aptasensors based on nanomaterials have shown great potential for the monitoring and detection of antibiotic residues, and extensive research is being conducted in this field.

TRENDS IN FOOD SCIENCE & TECHNOLOGY (2022)

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Article Biochemical Research Methods

Engineering a Synthetic Dopamine-Responsive Riboswitch for In Vitro Biosensing

Svetlana V. Harbaugh et al.

Summary: By converting RNA aptamers into functional riboswitches, direct detection and control of gene expression for analytes such as dopamine can be achieved. This research is expected to have broad applicability for the conversion of in vitro-generated RNA aptamers into fieldable molecular diagnostics.

ACS SYNTHETIC BIOLOGY (2022)

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Article Microbiology

One-Pot Isothermal LAMP-CRISPR-Based Assay for Klebsiella pneumoniae Detection

Xiaotong Qiu et al.

Summary: This study developed a simple, rapid, and highly specific method for detecting Klebsiella pneumoniae using the CRISPR-top assay. The assay can accurately identify K. pneumoniae within 60 minutes at a constant temperature, without requiring specific instruments.

MICROBIOLOGY SPECTRUM (2022)

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Article Chemistry, Analytical

Multiple crRNAs-assisted CRISPR/Cas12a assay targeting cytochrome b gene for amplification-free detection of meat adulteration

Yanju Chen et al.

Summary: This study demonstrates that combining multiple crRNAs in the CRISPR/Cas12a system can enhance detection sensitivity and establishes a multiple crRNAs enhanced CRISPR (meCRISPR) method for meat adulteration identification.

ANALYTICA CHIMICA ACTA (2022)

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Article Biophysics

Aptamer-based Cas14a1 biosensor for amplification-free live pathogenic detection

Yangdao Wei et al.

Summary: A new platform for the detection of live Staphylococcus aureus (S. aureus) based on an Aptamer-based Cas14a1 Biosensor (ACasB) has been developed. This platform shows high specificity and sensitivity, and can be applied in food safety and environmental monitoring.

BIOSENSORS & BIOELECTRONICS (2022)

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Review Food Science & Technology

Advances in magnetic nanoparticles for the separation of foodborne pathogens: Recognition, separation strategy, and application

Fangbin Xiao et al.

Summary: Foodborne pathogens contamination is a major source of food safety problems. Magnetic separation strategy based on magnetic nanoparticles (MNPs) is considered an effective tool for rapid separation and enrichment of foodborne pathogens in food. This study comprehensively reviews the development of MNPs in the separation of foodborne pathogens, discusses the factors affecting separation, and explores the challenges and prospects of MNPs for the analysis of foodborne pathogens.

COMPREHENSIVE REVIEWS IN FOOD SCIENCE AND FOOD SAFETY (2022)

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Article Chemistry, Applied

CRISPR-Cas12a based fluorescence assay for organophosphorus pesticides in agricultural products

Ruijie Fu et al.

Summary: In this study, a sensitive fluorescent assay for detection of organophosphorus pesticides (OPs) based on activating the CRISPR-Cas12a system is proposed. By utilizing the catalytic properties of acetylcholinesterase (AChE) and DNAzyme, the fluorescence signal is amplified for OPs detection. The method shows low detection limits and holds great potential for application in agricultural products.

FOOD CHEMISTRY (2022)

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Article Chemistry, Applied

Electrochemiluminescence covalent organic framework coupling with CRISPR/Cas12a-mediated biosensor for pesticide residue detection

Yan Li et al.

Summary: An electrochemiluminescence (ECL) covalent organic framework (COF) based-biosensor was developed for trace pesticide detection. The sensor converts pesticides into activator DNA, triggering CRISPR/Cas12a for signal accumulation. The COF also exhibited stable and strong ECL properties.

FOOD CHEMISTRY (2022)

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Article Chemistry, Analytical

CRISPR/dCas9-surface-enhanced Raman scattering for the detection of drug resistance gene macB

Yuwan Du et al.

Summary: Antibiotic overuse has led to new public health issues, and this paper presents an analysis method combining CRISPR and SERS technology to accurately detect the drug resistance gene macB, thereby improving prevention of gene contamination in food hygiene.

MICROCHIMICA ACTA (2022)

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Article Food Science & Technology

Recent advances on CRISPR/Cas system-enabled portable detection devices for on-site agri-food safety assay

Manyan Qiu et al.

Summary: Agri-food safety is a global concern, especially during the COVID-19 pandemic. This review summarizes the recent advances in CRISPR/Cas system-based biosensing technologies and their portable detection devices, as well as their applications in the field of agri-food safety. The integration of CRISPR/Cas systems-based biosensing technologies with portable detection devices shows great potential for on-site detection of agri-food contaminants.

TRENDS IN FOOD SCIENCE & TECHNOLOGY (2022)

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Article Chemistry, Analytical

Ultrafast visual nucleic acid detection with CRISPR/Cas12a and rapid PCR in single capillary

Rui Wang et al.

Summary: The proposed method integrates rapid PCR amplification and Cas12a cleavage, simplifying the operation and avoiding contamination. It demonstrates high detection sensitivity and reliability, and has broad application prospects in fields such as food safety and clinical diagnosis, offering a potential solution for rapid on-site testing.

SENSORS AND ACTUATORS B-CHEMICAL (2021)

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Review Food Science & Technology

Advances in typing and identification of foodborne pathogens

Xinyue Wei et al.

Summary: This paper discusses the importance of detecting foodborne pathogens, highlighting the limitations of traditional methods and advancements in new technologies. Various potential subtyping methods are proposed for improving foodborne pathogen detection.

CURRENT OPINION IN FOOD SCIENCE (2021)

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Article Chemistry, Multidisciplinary

Holographic Optical Tweezers and Boosting Upconversion Luminescent Resonance Energy Transfer Combined Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas12a Biosensors

Cheng-Yu Li et al.

Summary: The CRISPR/Cas12a system offers an alternative opportunity for designing fluorescence biosensors with outstanding precision in target recognition. By utilizing collateral cleavage and representative transduction pathways, the system can extend sensing coverage and improve assay sensitivity. The developed biosensors not only exhibit ultrasensitive behavior towards non-nucleic acid analytes but also show practical application capacity in real samples.

ACS NANO (2021)

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Article Biochemistry & Molecular Biology

Amplification-free detection of SARS-CoV-2 with CRISPR-Cas13a and mobile phone microscopy

Parinaz Fozouni et al.

Summary: This study developed an amplification-free CRISPR-Cas13a assay for direct detection of SARS-CoV-2, showing rapid, highly sensitive, and mobile phone-based readout capabilities.

CELL (2021)

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Article Biophysics

A chemical-enhanced system for CRISPR-Based nucleic acid detection

Zihan Li et al.

Summary: By optimizing reaction chemistry parameters, the development of a Chemical Enhanced CRISPR Detection system (CECRID) has shown improved detection sensitivity and robustness for viral pathogens. Experimental results demonstrate that CECRID can more effectively conduct nucleic acid detection in the context of the COVID-19 pandemic.

BIOSENSORS & BIOELECTRONICS (2021)

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Article Chemistry, Applied

RPA-Cas12a-FS: A frontline nucleic acid rapid detection system for food safety based on CRISPR-Cas12a combined with recombinase polymerase amplification

Hua Liu et al.

Summary: This study developed a detection technique by combining recombinase polymerase amplification with CRISPR-Cas12a for food safety, which can rapidly, simply, and highly sensitively detect foodborne pathogenic bacteria, genetically modified crops, and meat adulteration without requiring technical expertise or equipment.

FOOD CHEMISTRY (2021)

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Article Biophysics

Light-up RNA aptamer signaling-CRISPR-Cas13a-based mix-and-read assays for profiling viable pathogenic bacteria

Ting Zhang et al.

Summary: This study developed a new detection system that can accurately distinguish viable pathogenic bacteria without the need for reverse transcription, nucleic acid amplification, or labeled substrates. By utilizing CRISPR-Cas13a and a light-up RNA aptamer, it enables rapid detection and precise quantification of viable pathogens.

BIOSENSORS & BIOELECTRONICS (2021)

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Article Chemistry, Analytical

Ultrasensitive detection of pathogenic bacteria by CRISPR/Cas12a coupling with a primer exchange reaction

Shengjun Bu et al.

Summary: This study developed an electrochemical biosensor based on CRISPR/Cas12a and primer exchange reaction (PER) for detecting pathogenic bacteria without the need for nucleic acid extraction. The sensor quantified Escherichia coli O157:H7 concentration from 10 to 106 CFU mL-1, with a detection limit of 19 CFU mL-1, and successfully detected target bacteria in milk samples. This biosensor has potential as a new tool for point-of-care applications in food safety.

SENSORS AND ACTUATORS B-CHEMICAL (2021)

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Review Food Science & Technology

Genomics-based approaches to identify and predict the health-promoting and safety activities of promising probiotic strains-A probiogenomics review

Cecilia Castro-Lopez et al.

Summary: The clinical evidence supporting the beneficial effects of probiotics in consumer's health and wellness has increased over the past two decades, leading to the production of different probiotic foods and strains by the food and pharmaceutical industries. Comprehensive trials are necessary to characterize probiotic candidates, including their resistance to physiological barriers, inhibition of pathogens, adherence to mucus, and other properties. Further research is needed to clarify the metabolic capabilities, adaptation features, physiological functions, safety, and molecular mechanisms underlying the functional activities of probiotics. Genomics-based approaches, such as probiogenomics, have been successfully applied to study current probiotics and identify new potential candidates with health-promoting and safety properties.

TRENDS IN FOOD SCIENCE & TECHNOLOGY (2021)

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Article Food Science & Technology

Application progress of microfluidics-integrated biosensing platforms in the detection of foodborne pathogens

XuRan Fu et al.

Summary: Microfluidics-integrated biosensors offer advantages such as miniaturization, high throughput, rapid integration, and less consumables, making them a promising tool for food safety testing. Recent advancements in the application of microfluidics for detecting foodborne pathogens have shown potential for effective, rapid, and accurate detection. Future research should focus on improving data storage, transmission, and detection sensitivity for enhanced field testing capabilities.

TRENDS IN FOOD SCIENCE & TECHNOLOGY (2021)

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Review Chemistry, Multidisciplinary

COVID-19 Antibody Tests and Their Limitations

Guoqiang Liu et al.

Summary: COVID-19 pandemic is a global health crisis with over 2 million deaths, where the main diagnostic methods are nucleic acid PCR tests, viral antigen tests, and human antibody tests. The limitations of antibody tests include inaccuracies due to various factors such as testing windows, individual variances, and fluctuation in antibody levels.

ACS SENSORS (2021)

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Article Biophysics

A reversible valve-assisted chip coupling with integrated sample treatment and CRISPR/Cas12a for visual detection of Vibrio parahaemolyticus

Hui Wu et al.

Summary: A rapid and low-cost detection method for V. parahaemolyticus using a chip and CRISPR/Cas12a was established, allowing for detection within 50 minutes with high sensitivity. The method showed great feasibility for real samples detection and has potential applications in food safety analysis and clinical diagnostics, especially in resource-limited areas.

BIOSENSORS & BIOELECTRONICS (2021)

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Article Chemistry, Multidisciplinary

CRISPR/Cas12a-Based Versatile Method for Checking Quantitative Polymerase Chain Reaction Samples with Cycles of Threshold Values in the Gray Zone

Yanju Chen et al.

Summary: qPCR gray zone testing results in uncertainty, while CRISPR technology can enhance detection signal sensitivity. A CRISPR-based checking method is proposed to solve gray zone problems, effectively eliminating uncertainty in sample classification without increasing workload in PCR detection.

ACS SENSORS (2021)

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Article Chemistry, Analytical

A novel colorimetric aptasensor for ultrasensitive detection of aflatoxin M1 based on the combination of CRISPR-Cas12a, rolling circle amplification and catalytic activity of gold nanoparticles

Khalil Abnous et al.

Summary: A highly sensitive colorimetric aptasensor was developed for the detection of AFM(1) using CRISPR-Cas12a, RCA, and AuNPs. The sensor showed high selectivity towards AFM(1) with a detection limit as low as 0.05 ng/L, and was able to sensitively identify AFM(1) in spiked milk samples.

ANALYTICA CHIMICA ACTA (2021)

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Article Biophysics

?Aptamer-locker? DNA coupling with CRISPR/Cas12a-guided biosensing for high-efficiency melamine analysis

Bin Qiao et al.

Summary: A method combining "aptamer-locker" DNA with CRISPR/Cas12a-based biosensing was developed for sensitive and rapid melamine analysis, achieving good detection performance with a low limit of detection. The approach demonstrated high sensitivity, selectivity, and real-time analysis capabilities for detecting melamine in dairy products.

BIOSENSORS & BIOELECTRONICS (2021)

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Article Chemistry, Multidisciplinary

CRISPR-Cas12a-Powered Dual-Mode Biosensor for Ultrasensitive and Cross-validating Detection of Pathogenic Bacteria

Long Ma et al.

Summary: The study proposed a CRISPR-Cas12a-powered dual-mode biosensor for ultrasensitive and cross-validating detection of pathogenic bacteria, with satisfactory selectivity for Salmonella and capabilities in food sample detection. The technology expands the reach of CRISPR-Cas system in biosensing and provides a general method for bacteria sensing with desirable sensitivity, specificity, and cross-validating capacity.

ACS SENSORS (2021)

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Editorial Material Food Science & Technology

Editorial overview: Food safety

Tian Ding

CURRENT OPINION IN FOOD SCIENCE (2021)

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Review Chemistry, Physical

Structure-Switching RNAs: From Gene Expression Regulation to Small Molecule Detection

Claire Husser et al.

Summary: RNA plays a crucial role in cell life, particularly in gene expression regulation. Riboswitches, as cis-acting molecules, are able to specifically sense a target ligand and control the expression of downstream genes without the need for cellular factors, making them highly portable and potentially useful in various applications such as gene therapy and biosensing of small molecules.

SMALL STRUCTURES (2021)

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Article Chemistry, Multidisciplinary

Element probe based CRISPR/Cas14 bioassay for non-nucleic-acid targets

Jianyu Hu et al.

Summary: A novel element probe based CRISPR/Cas14 detection platform was proposed in this study, allowing sensitive and quantitative detection of non-nucleic-acid targets such as trace aqueous ampicillin within 45 minutes at room temperature. The method showed excellent performance in anti-interference tests and complex matrix detection, with a low detection limit of 2.06 nM.

CHEMICAL COMMUNICATIONS (2021)

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Article Chemistry, Multidisciplinary

A highly sensitive and selective fluoride sensor based on a riboswitch-regulated transcription coupled with CRISPR-Cas13a tandem reaction

Yuan Ma et al.

Summary: A portable fluoride sensor based on fluoride riboswitch and CRISPR-Cas13 signal amplification has been developed, allowing for the quantitative detection of fluoride in aqueous solution with high sensitivity and selectivity. This sensor shows potential for on-site and real-time environmental monitoring and diagnostics.

CHEMICAL SCIENCE (2021)

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Article Chemistry, Multidisciplinary

Clustered Regularly Interspaced Short Palindromic Repeats/Cas9-Mediated Lateral Flow Nucleic Acid Assay

Xusheng Wang et al.

ACS NANO (2020)

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Article Chemistry, Analytical

A novel RNA aptamer-modified riboswitch as chemical sensor

Jing Wang et al.

ANALYTICA CHIMICA ACTA (2020)

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Article Chemistry, Analytical

CRISPR-Cas9 Triggered Two-Step Isothermal Amplification Method for E. coli O157:H7 Detection Based on a Metal-Organic Framework Platform

Xuan Sun et al.

ANALYTICAL CHEMISTRY (2020)

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Review Microbiology

Evolutionary classification of CRISPR-Cas systems: a burst of class 2 and derived variants

Kira S. Makarova et al.

NATURE REVIEWS MICROBIOLOGY (2020)

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Article Multidisciplinary Sciences

Massively multiplexed nucleic acid detection with Cas13

Cheri M. Ackerman et al.

NATURE (2020)

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Article Biophysics

End-point dual specific detection of nucleic acids using CRISPR/Cas12a based portable biosensor

Hui Wu et al.

BIOSENSORS & BIOELECTRONICS (2020)

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Review Biochemistry & Molecular Biology

A review: Recent advances in ultrasensitive and highly specific recognition aptasensors with various detection strategies

Shu-Rong Yan et al.

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2020)

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Article Nanoscience & Nanotechnology

Magnetic Bead-Quantum Dot (MB-Qdot) Clustered Regularly Interspaced Short Palindromic Repeat Assay for Simple Viral DNA Detection

Mengdi Bao et al.

ACS APPLIED MATERIALS & INTERFACES (2020)

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Article Biophysics

Point-of-care CRISPR/Cas nucleic acid detection: Recent advances, challenges and opportunities

Jeanne E. van Dongen et al.

BIOSENSORS & BIOELECTRONICS (2020)

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Article Biophysics

A lateral flow strip combined with Cas9 nickase-triggered amplification reaction for dual food-borne pathogen detection

Luying Wang et al.

BIOSENSORS & BIOELECTRONICS (2020)

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Article Multidisciplinary Sciences

Barcoded microbial system for high-resolution object provenance

Jason Qian et al.

SCIENCE (2020)

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Article Biochemistry & Molecular Biology

SPRINT: a Cas13a-based platform for detection of small molecules

Roman S. Iwasaki et al.

NUCLEIC ACIDS RESEARCH (2020)

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Review Biochemistry & Molecular Biology

Molecular Mechanisms of RNA Targeting by Cas13-containing Type VI CRISPR-Cas Systems

Mitchell R. O'Connell

JOURNAL OF MOLECULAR BIOLOGY (2019)

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Article Biochemistry & Molecular Biology

Defining the seed sequence of the Cas12b CRISPR-Cas effector complex

Ishita Jain et al.

RNA BIOLOGY (2019)

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Review Cell Biology

The next generation of CRISPR-Cas technologies and applications

Adrian Pickar-Oliver et al.

NATURE REVIEWS MOLECULAR CELL BIOLOGY (2019)

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Article Multidisciplinary Sciences

Plug-and-play metabolic transducers expand the chemical detection space of cell-free biosensors

Peter L. Voyvodic et al.

NATURE COMMUNICATIONS (2019)

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Article Biochemical Research Methods

HOLMESv2: A CRISPR-Cas12b-Assisted Platform for Nucleic Acid Detection and DNA Methylation Quantitation

Linxian Li et al.

ACS SYNTHETIC BIOLOGY (2019)

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Review Engineering, Electrical & Electronic

Recent Advances and Progress in Development of the Field Effect Transistor Biosensor: A Review

Tanu Wadhera et al.

JOURNAL OF ELECTRONIC MATERIALS (2019)

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Article Multidisciplinary Sciences

A CRISPR-Cas12a-derived biosensing platform for the highly sensitive detection of diverse small molecules

Mindong Liang et al.

NATURE COMMUNICATIONS (2019)

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Article Biotechnology & Applied Microbiology

CDetection: CRISPR-Cas12b-based DNA detection with sub-attomolar sensitivity and single-base specificity

Fei Teng et al.

GENOME BIOLOGY (2019)

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Review Chemistry, Analytical

Noble Metal Nanoparticles-Based Colorimetric Biosensor for Visual Quantification: A Mini Review

Lu Yu et al.

CHEMOSENSORS (2019)

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Article Chemistry, Multidisciplinary

Programmable autonomous synthesis of single-stranded DNA

Jocelyn Y. Kishi et al.

NATURE CHEMISTRY (2018)

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Letter Cell Biology

CRISPR-Cas12a has both cis- and trans-cleavage activities on single-stranded DNA

Shi-Yuan Li et al.

CELL RESEARCH (2018)

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Article Multidisciplinary Sciences

CRISPR-Cas12a target binding unleashes indiscriminate single-stranded DNase activity

Janice S. Chen et al.

SCIENCE (2018)

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Article Multidisciplinary Sciences

Multiplexed and portable nucleic acid detection platform with Cas13, Cas12a, and Csm6

Jonathan S. Gootenberg et al.

SCIENCE (2018)

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