Chimaeras, complementation, and controlling the male germline

Article Multidisciplinary Sciences

Human blastoids model blastocyst development and implantation

Harunobu Kagawa et al.

Summary: Human pluripotent stem cells cultured in specific conditions can efficiently form blastoids resembling blastocyst-stage analogues, providing a faithful model for investigating human implantation and development.

NATURE (2022)

添加到收藏夹

Article Multidisciplinary Sciences

Healthy cloned offspring derived from freeze-dried somatic cells

Sayaka Wakayama et al.

Summary: Research shows that freeze-dried somatic cells can produce healthy cloned mice, which is important for the establishment of alternative, cheaper, and safer liquid nitrogen-free bio-banking solutions.

NATURE COMMUNICATIONS (2022)

添加到收藏夹

Article Cell & Tissue Engineering

Exclusive generation of rat spermatozoa in sterile mice utilizing blastocyst complementation with pluripotent stem cells

Joel Zvick et al.

Summary: This study successfully achieved complementation of functional spermatozoa in adult chimeras through the technique of blastocyst complementation. Using mouse and rat pluripotent stem cells, the researchers were able to generate chimeras with either species-specific sperm or interspecies sperm capable of fertilization. Single-cell RNA sequencing was used to study spermatogenesis in the chimeric testis. The findings of this study have important implications for germ cell research and conservation efforts for endangered animal species.

STEM CELL REPORTS (2022)

添加到收藏夹

Review Agriculture, Multidisciplinary

Synergistic power of genomic selection, assisted reproductive technologies, and gene editing to drive genetic improvement of cattle

Maci L. Mueller et al.

Summary: Genetic improvement of cattle is crucial for the sustainable development of animal agriculture worldwide. Reproductive and molecular biotechnologies are used to enhance genetic progress, and gene editing is a recent tool that allows precise modification of the genetic code. Integration of gene editing into conventional cattle breeding programs is necessary for its effectiveness.

CABI AGRICULTURE & BIOSCIENCE (2022)

添加到收藏夹

Article Reproductive Biology

Generation of chimeric mice with spermatozoa fully derived from embryonic stem cells using a triple-target CRISPR method for Nanos3

Kento Miura et al.

Summary: A CRISPR/Cas9 system utilizing three guide RNAs targeting Nanos3 was able to generate chimeric mice with spermatozoa fully derived from ESCs, confirming that Dnmt3b is not essential for male germ cell development.

BIOLOGY OF REPRODUCTION (2021)

添加到收藏夹

Article Developmental Biology

Pluripotent stem cells related to embryonic disc exhibit common self-renewal requirements in diverse livestock species

Masaki Kinoshita et al.

Summary: This study successfully established pluripotent stem cell lines from porcine, ovine, and bovine embryos without using feeder cells or serum factors. The cells showed differentiation in teratomas and in vitro, with global transcriptome analyses revealing commonality in transcription factor expression across the three species. The research also demonstrated the generation of cloned porcine fetuses using genetically modified AFX stem cells through nuclear transfer.

DEVELOPMENT (2021)

添加到收藏夹

Article Biochemistry & Molecular Biology

Testes of DAZL null neonatal sheep lack prospermatogonia but maintain normal somatic cell morphology and marker expression

Zachariah L. McLean et al.

Summary: By disrupting the RNA-binding protein DAZL and creating germ cell-deficient host animals, researchers successfully generated host animals lacking germ cells, paving the way for rapid genetic improvement in livestock breeding.

MOLECULAR REPRODUCTION AND DEVELOPMENT (2021)

添加到收藏夹

Article Multidisciplinary Sciences

Blastocyst complementation using Prdm14-deficient rats enables efficient germline transmission and generation of functional mouse spermatids in rats

Toshihiro Kobayashi et al.

Summary: The study demonstrates efficient germline transmission of difficult PSCs by complementing blastocysts from germline-deficient Prdm14 knockout rats, showing the potential for creating genetically modified animals and cross-species germline transmission.

NATURE COMMUNICATIONS (2021)

添加到收藏夹

Article Cell & Tissue Engineering

Transient suppression of transplanted spermatogonial stem cell differentiation restores fertility in mice

Yoshiaki Nakamura et al.

Summary: Tissue stem cells have the ability to regenerate host tissue following transplantation, but the dynamics of donor stem cells during regeneration are still largely unknown. Research on mouse spermatogonial stem cells showed that only a small fraction can persist and regenerate in the long term, while the rest are lost through differentiation and cell death. By transiently suppressing differentiation, repopulation efficiency can be increased to restore the fertility of infertile hosts.

CELL STEM CELL (2021)

添加到收藏夹

Review Agriculture, Dairy & Animal Science

Spermatogonial Stem Cell Transplantation in Large Animals

Xin Zhao et al.

Summary: Spermatogonial stem cell transplantation (SSCT) can restore male fertility by transferring germline between donor and recipient males. From an agricultural perspective, SSCT could be an important next-generation reproductive and breeding tool in livestock production. Current SSCT approaches in large animals are inefficient, and further investigation is needed for many technical details.

ANIMALS (2021)

添加到收藏夹

Review Developmental Biology

The road to generating transplantable organs: from blastocyst complementation to interspecies chimeras

Canbin Zheng et al.

Summary: Interspecies blastocyst complementation involves injecting donor stem cells into a host embryo to compensate for missing organs or tissues. While it has been successful between closely related species, it presents greater challenges for species that are further apart evolutionarily due to xenogeneic barriers. Overcoming these barriers is crucial for successful interspecies organ generation.

DEVELOPMENT (2021)

添加到收藏夹

Article Cell & Tissue Engineering

Spermatogonial stem cell transplantation into nonablated mouse recipient testes

Hiroko Morimoto et al.

Summary: The study shows that recipient conditioning plays a significant role in the homing of spermatogonial stem cells (SSCs), with nonablated niches attracting SSCs more efficiently. In Cldn11-deficient mice without a blood-testis barrier (BTB), transplantation not only enhances donor colonization but also restores normal spermatogenesis, hinting at a potential new transplantation strategy for overcoming male infertility.

STEM CELL REPORTS (2021)

添加到收藏夹

Article Multidisciplinary Sciences

Autologous transplantation of spermatogonial stem cells restores fertility in congenitally infertile mice

Mito Kanatsu-Shinohara et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2020)

添加到收藏夹

Article Multidisciplinary Sciences

Donor-derived spermatogenesis following stem cell transplantation in sterile NANOS2 knockout males

Michela Ciccarelli et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2020)

添加到收藏夹

Article Developmental Biology

Derivation of sheep embryonic stem cells under optimized conditions

Marcela Vilarino et al.

REPRODUCTION (2020)

添加到收藏夹

Article Developmental Biology

Defined conditions for propagation and manipulation of mouse embryonic stem cells

Carla Mulas et al.

DEVELOPMENT (2019)

添加到收藏夹

Article Developmental Biology

The testicular soma of Tsc22d3 knockout mice supports spermatogenesis and germline transmission from spermatogonial stem cell lines upon transplantation

Hai Zhou et al.

GENESIS (2019)

添加到收藏夹

Article Cell Biology

Establishment of porcine and human expanded potential stem cells

Xuefei Gao et al.

NATURE CELL BIOLOGY (2019)

添加到收藏夹

Review Agriculture, Dairy & Animal Science

Manipulation of spermatogonial stem cells in livestock species

Filipp Savvulidi et al.

JOURNAL OF ANIMAL SCIENCE AND BIOTECHNOLOGY (2019)

添加到收藏夹

Article Multidisciplinary Sciences

Mammalian germ cells are determined after PGC colonization of the nascent gonad

Peter K. Nicholls et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2019)

添加到收藏夹

Review Reproductive Biology

Spermatogonial stem cells

Hiroshi Kubota et al.

BIOLOGY OF REPRODUCTION (2018)

添加到收藏夹

Article Biochemistry & Molecular Biology

TALEN-mediated gene targeting in porcine spermatogonia

Lin Tang et al.

MOLECULAR REPRODUCTION AND DEVELOPMENT (2018)

添加到收藏夹

Article Biochemistry & Molecular Biology

Long-term culture of undifferentiated spermatogonia isolated from immature and adult bovine testes

Suyatno et al.

MOLECULAR REPRODUCTION AND DEVELOPMENT (2018)

添加到收藏夹

Article Multidisciplinary Sciences

Blastocyst-like structures generated solely from stem cells

Nicolas C. Rivron et al.

NATURE (2018)

添加到收藏夹

Article Multidisciplinary Sciences

Efficient derivation of stable primed pluripotent embryonic stem cells from bovine blastocysts

Yanina Soledad Bogliotti et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2018)

添加到收藏夹

Article Multidisciplinary Sciences

Embryos and embryonic stem cells from the white rhinoceros

Thomas B. Hildebrandt et al.

NATURE COMMUNICATIONS (2018)

添加到收藏夹

Article Multidisciplinary Sciences

An interspecies barrier to tetraploid complementation and chimera formation

Tomoyuki Yamaguchi et al.

SCIENTIFIC REPORTS (2018)

添加到收藏夹

Article Cell & Tissue Engineering

Long-Term Propagation of Porcine Undifferentiated Spermatogonia

Pengfei Zhang et al.

STEM CELLS AND DEVELOPMENT (2017)

添加到收藏夹

Article Multidisciplinary Sciences

Generation of germline ablated male pigs by CRISPR/Cas9 editing of the NANOS2 gene

Ki-Eun Park et al.

SCIENTIFIC REPORTS (2017)

添加到收藏夹

Article Cell & Tissue Engineering

Glycolysis-Optimized Conditions Enhance Maintenance of Regenerative Integrity in Mouse Spermatogonial Stem Cells during Long-Term Culture

Aileen R. Helsel et al.

STEM CELL REPORTS (2017)

添加到收藏夹

Article Reproductive Biology

Fertility of Male Germline Stem Cells Following Spermatogonial Transplantation in Infertile Mouse Models

Mito Kanatsu-Shinohara et al.

BIOLOGY OF REPRODUCTION (2016)

添加到收藏夹

Article Developmental Biology

Exclusive transmission of the embryonic stem cell-derived genome through the mouse germline

Frank Koentgen et al.

GENESIS (2016)

添加到收藏夹

Article Multidisciplinary Sciences

Generation of exogenous germ cells in the ovaries of sterile NANOS3-null beef cattle

Atsushi Ideta et al.

SCIENTIFIC REPORTS (2016)

添加到收藏夹

Article Cell Biology

Targeted Germline Modifications in Rats Using CRISPR/Cas9 and Spermatogonial Stem Cells

Karen M. Chapman et al.

CELL REPORTS (2015)

添加到收藏夹

Article Cell & Tissue Engineering

Genome Editing in Mouse Spermatogonial Stem Cell Lines Using TALEN and Double-Nicking CRISPR/Cas9

Takuya Sato et al.

STEM CELL REPORTS (2015)

添加到收藏夹

Article Genetics & Heredity

Isolation, identification, and culture of goat spermatogonial stem cells using c-kit and PGP9.5 markers

Banafsheh Heidari et al.

JOURNAL OF ASSISTED REPRODUCTION AND GENETICS (2012)

添加到收藏夹

Article Reproductive Biology

Serum- and Feeder-Free Culture of Mouse Germline Stem Cells

Mito Kanatsu-Shinohara et al.

BIOLOGY OF REPRODUCTION (2011)

添加到收藏夹

Article Developmental Biology

Complex ovarian defects lead to infertility in Etv5-/- female mice

Jinwon Eo et al.

MOLECULAR HUMAN REPRODUCTION (2011)

添加到收藏夹

Article Cell & Tissue Engineering

A Murine ESC-like State Facilitates Transgenesis and Homologous Recombination in Human Pluripotent Stem Cells

Christa Buecker et al.

CELL STEM CELL (2010)

添加到收藏夹

Article Cell & Tissue Engineering

Effect of Microinjection of a Single IVF-Derived Blastomere on the Development of Cloned Embryos at the Eight-Cell Stage in Bovine

Xiuping Chen et al.

CELLULAR REPROGRAMMING (2010)

添加到收藏夹

Article Multidisciplinary Sciences

Production of knockout mice by random or targeted mutagenesis in spermatogonial stem cells

Mito Kanatsu-Shinohara et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2006)

添加到收藏夹

Article Reproductive Biology

Sex differentiation and germ cell production in chimeric pigs produced by inner cell mass injection into blastocysts

H Nagashima et al.

BIOLOGY OF REPRODUCTION (2004)

添加到收藏夹

Article Multidisciplinary Sciences

Growth factors essential for self-renewal and expansion of mouse spermatogonial stem cells

H Kubota et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2004)

添加到收藏夹

Article Obstetrics & Gynecology

Restoration of fertility in infertile mice by transplantation of cryopreserved male germline stem cells

M Kanatsu-Shinohara et al.

HUMAN REPRODUCTION (2003)

添加到收藏夹

Article Reproductive Biology

Functional assessment of self-renewal activity of male germline stem cells following cytotoxic damage and serial transplantation

M Kanatsu-Shinohara et al.

BIOLOGY OF REPRODUCTION (2003)

添加到收藏夹

Article Multidisciplinary Sciences

Remodeling of the postnatal mouse testis is accompanied by dramatic changes in stem cell number and niche accessibility

T Shinohara et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2001)

添加到收藏夹

Chimaeras, complementation, and controlling the male germline

相关参考文献

导出引文

分享论文