Tuberculosis Epidemiology and Spatial Ecology at the Cattle-Wild Boar Interface in Northern Spain

Tuberculosis (TB) is a contagious chronic disease due to infection with Mycobacterium tuberculosis complex (MTC) bacteria. Monitoring of wildlife, especially potential reservoirs, is important for detecting changes in disease occurrence and assessing the impact of interventions. Here, we examined whether wild boar (Sus scrofa) may contribute to the re-emergence of TB in Asturias (10,604 km(2)), northern Spain. Although this province was declared free of TB in cattle in November 2021, MTC bacteria remain prevalent in several hotspots, with the European badger (Meles meles) suggested as a TB potential wild reservoir. Drawing on data from the Spanish National Bovine Tuberculosis Eradication Program and the Government of the Principality of Asturias covering the period 2014-2020, we analyzed the prevalence of TB in cattle and wild boar in this region. In hotspots (592 km(2)), we also investigated the ranging behavior and habitat use of five cows that belonged to farms with a history of TB and six trapped sympatric wild boar. During the observation period, TB prevalence was 0.14% among cattle overall and 0.13-0.41% in hotspots, which was much lower than the prevalence in wild boar, which was 3.15% overall and 5.23-5.96% in hotspots. Infected cattle and infected wild boar in hotspots shared the same strains of M. bovis, and GPS tracking showed spatiotemporal overlap between the species, mainly around pastures during sunrise (06:00-07:00 h) and sunset (19:00-20:00 h). Our results suggest that in addition to cattle and badgers, wild boar possibly help maintain TB in northern Spain, increasing the host richness that influences TB transmission risk in the area, which should be taken into account in monitoring and eradication efforts.

Automated summary

By analyzing the prevalence of tuberculosis in cattle and wild boar as well as their ranging behavior and habitat use in Asturias, northern Spain from 2014 to 2020, this study found that wild boar may contribute to the re-emergence of TB in the region. Infected wild boar shared the same strains of M. bovis with infected cattle, and their spatiotemporal overlap around pastures increased the transmission risk of TB. These findings are important for monitoring and eradication efforts against tuberculosis.