The quite low cross-reactivity of Kawatsu?s anti-tetrodotoxin monoclonal antibody to 5,6,11-trideoxytetrodotoxin, 11-nortetrodotoxin-6(S)-ol, and 11-oxotetrodotoxin, the major tetrodotoxin analogues in pufferfish

The monoclonal antibody against tetrodotoxin (TTX), prepared by Kawatsu et al. (1997), has been used in several TTX-related studies. Herein, we confirmed the quite low cross-reactivity of this antibody to three major TTX analogues in pufferfish using competitive ELISA: 5,6,11-trideoxyTTX (<2.2%), 11-norTTX-6(S)-ol (<0.3%), and 11-oxoTTX (<1.5%), with reactivity against TTX being 100%. We further confirmed that the presence of these analogues did not cause a marked overestimation of TTX in pufferfish extracts using competitive ELISA.

Automated summary

This study confirmed the low cross-reactivity of a monoclonal antibody against tetrodotoxin (TTX) to three major TTX analogues in pufferfish using competitive ELISA. The reactivity against TTX was 100%, while the cross-reactivity to 5,6,11-trideoxyTTX, 11-norTTX-6(S)-ol, and 11-oxoTTX was less than 2.2%, 0.3%, and 1.5% respectively. Additionally, the presence of these analogues did not cause overestimation of TTX in pufferfish extracts using competitive ELISA.