4.2 Review

Three-Dimensional Cell Culture System for Tendon Tissue Engineering

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Summary: The study verified the potential of the decellularized umbilical cord Wharton jelly scaffold in promoting tendon healing and enhancing biomechanical strength in a rabbit model of acute rotator cuff tendon defect.

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Hierarchical ultrastructure: An overview of what is known about tendons and future perspective for tendon engineering

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Summary: This review summarizes recent research on the structure and function of the extracellular matrix (ECM) components of tendons and highlights the application of multiple detection methodologies concerning the structure of ECMs. The focus is on macro to micro detection methods for tendons, and current techniques for evaluating the extracellular matrix of tendons at the micro level are introduced in detail. The review also emphasizes future ECM detection methods and the potential for fabricating biomimetic tendons.

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Co-Electrospun Silk Fibroin and Gelatin Methacryloyl Sheet Seeded with Mesenchymal Stem Cells for Tendon Regeneration

Yumeng Xue et al.

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A Natural Hydrogel with Prohealing Properties Enhances Tendon Regeneration

Ruyi Dang et al.

Summary: This study investigates the use of a hydrogel derived from the skin secretion of Andrias davidianus to address the challenges of tendon regeneration and reduction of peritendinous adhesion. The hydrogel contains cytokines that promote tendon healing and has controllable microstructures. In both in vitro and in vivo experiments, the hydrogel demonstrated its ability to promote tendon healing, reduce peritendinous adhesion, and exhibit antioxidant and antibacterial characteristics. The results suggest that the SSAD-derived hydrogel may be a feasible biomaterial for tendon repair in the future.
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3D bioprinting of multilayered scaffolds with spatially differentiated ADMSCs for rotator cuff tendon-to-bone interface regeneration

Xiping Jiang et al.

Summary: This study presents a novel therapeutic method that combines three-dimensional (3D) bioprinting and melt electrospinning writing techniques to regenerate the tendon-to-bone interface. The use of 3D-bioprinted pre-differentiated autologous adipose-derived mesenchymal stem cells (ADMSC) generated biomimetic multilayered scaffolds that recapitulated the compositional and cellular structures of the interface. In vivo studies demonstrated the superior histological score and improved collagen organization of the scaffold with spatially differentiated autologous ADMSCs.

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Identification and Distinction of Tenocytes and Tendon-Derived Stem Cells

Yuange Li et al.

Summary: The discovery of tendon-derived stem cells offers a new perspective for treating tendon injuries, but the lack of specific markers poses obstacles in in vitro studies. This review summarizes and compares the characteristics of tenocytes and TDSCs, and suggests a potential marker selection for identification in the future. Further exploration of biomarker mechanisms in vivo and the discovery of more specific markers are of profound significance.

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Summary: Low cell engraftment and viability due to oxygen and nutrient depletion are challenges in tissue engineering. Fibroblast therapy is considered promising, especially with the unique properties of three-dimensional fibroblasts. Strategies for macrophage polarization offer possibilities for wound healing, but efficacy and safety concerns remain.

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An anisotropic nanocomposite hydrogel guides aligned orientation and enhances tenogenesis of human tendon stem/progenitor cells

Yichi Xu et al.

Summary: A magnetically-responsive nanocomposite hydrogel composed of collagen type I and aligned iron oxide nanoparticles was investigated for potential application in tendon tissue engineering. Compared to control groups, this novel hydrogel enhanced the morphology and proliferation of tendon cells, as well as promoted the upregulation of tendon gene markers.

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Maria Rita Citeroni et al.

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Giuseppe Filardo et al.

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Platelet-Rich Plasma: Review of Current Literature on its Use for Tendon and Ligament Pathology

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Combination of biochemical and mechanical cues for tendon tissue engineering

Stefano Testa et al.

JOURNAL OF CELLULAR AND MOLECULAR MEDICINE (2017)

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The microstructure and micromechanics of the tendon-bone insertion

L. Rossetti et al.

NATURE MATERIALS (2017)

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Spheroid formation and modulation of tenocyte-specific gene expression under simulated microgravity

Armin Kraus et al.

MLTJ-MUSCLES LIGAMENTS AND TENDONS JOURNAL (2017)

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Tendon-derived progenitor cells improve healing of collagenase-induced flexor tendinitis

Sushmitha S. Durgam et al.

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In vitro two-dimensional and three-dimensional tenocyte culture for tendon tissue engineering

Yiwei Qiu et al.

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Geoffroy Nourissat et al.

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A 3D bioprinted complex structure for engineering the muscle-tendon unit

Tyler K. Merceron et al.

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The guidance of stem cell differentiation by substrate alignment and mechanical stimulation

Siddarth D. Subramony et al.

BIOMATERIALS (2013)

Review Cell & Tissue Engineering

Engineering tendon and ligament tissues: present developments towards successful clinical products

Marcia T. Rodrigues et al.

JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE (2013)

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LLLT improves tendon healing through increase of MMP activity and collagen synthesis

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LASERS IN MEDICAL SCIENCE (2013)

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Spheroid culture as a tool for creating 3D complex tissues

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Tendon Healing: Repair and Regeneration

Pramod B. Voleti et al.

ANNUAL REVIEW OF BIOMEDICAL ENGINEERING, VOL 14 (2012)

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Characterization of differential properties of rabbit tendon stem cells and tenocytes

Jianying Zhang et al.

BMC MUSCULOSKELETAL DISORDERS (2010)

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Isolation and Characterization of Multipotent Rat Tendon-Derived Stem Cells

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Graham Riley

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Identification of tendon stem/progenitor cells and the role of the extracellular matrix in their niche

Yanming Bi et al.

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Tendon matrix composition and turnover in relation to functional requirements

Helen L. Birch

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RHEUMATOLOGY (2006)

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