Effects of novel variants in BMP15 gene on litter size in Mongolia and Ujimqin sheep breeds

Reproductive traits, such as ovulation rate and litter size, are important factors influencing the sheep industry. The bone morphogenetic protein 15 (BMP15) is a major gene affecting the reproductive traits in sheep, and multiple mutations in BMP15 gene could affect the ovulation rate and litter size in many sheep breeds, showing high breed specificity. However, identification of novel variations and seeking breed-specific markers associated with litter size in other sheep breeds are still important. In this study, we sequenced the BMP15 gene of Mongolia sheep, and 12 novel variants were detected by direct sequencing and whole-genome resequencing. Among them, the g.50985975 G > A polymorphism in intron and synonymous c.755 T > C (Leu252Pro) in exon 2 of BMP15 were significantly associated with the litter sizes of Mongolia ewes (P < 0.01 and P < 0.01, respectively), as well as the g.50988478C > A and g.50987863G > A in the promoter region of BMP15 were significantly associated with the litter sizes of Ujimqin ewes (P < 0.05 and P < 0.01, respectively). The c.755 T > C mutation is predicted to change the tertiary structure of BMP15. Our findings may provide potentially useful genetic markers for increasing litter size in sheep.(c) 2022 Elsevier Inc. All rights reserved.

Automated summary

Reproductive traits are important in the sheep industry, and the BMP15 gene plays a major role. This study identified 12 novel variants in the BMP15 gene of Mongolia sheep, and found significant associations between certain variants and litter size in Mongolia ewes and Ujimqin ewes. One of the mutations is predicted to affect the tertiary structure of BMP15. These findings may contribute to improving litter size in sheep.