Seasonal variations in the metabolomic profile of the ovarian follicle components in Italian Mediterranean Buffaloes

The aim of this work was to evaluate the seasonal effect on the metabolomic profile of the ovarian follicle in Italian Mediterranean buffalo to unravel the causes of the reduced competence during the non-breeding season (NBS). Samples of follicular fluid, follicular cells, cumulus cells and oocytes were collected from abattoir-derived ovaries during breeding season (BS) and NBS and analyzed by 1H Nuclear Magnetic Resonance. The Orthogonal Projections to Latent Structures of the Discriminant Analysis showed clear separation into seasonal classes and Variable Importance in Projection method identified differentially abundant metabolites between seasons. Seasonal differences were recorded in metabolite content in all analyzed components suggesting that the decreased oocyte competence during NBS may be linked to alteration of several metabolic pathways. The pathway enrichment analysis revealed that differences in the metabolites between the seasons were linked to glutathione, energy generating and amino acid metabolism and phospholipid biosynthesis. The current work allows the identification of potential positive competence markers in the follicular fluid as glutathione, glutamate, lactate and choline, and negative markers like leucine, isoleucine and beta-hydroxybutyrate. These results form a major basis to develop potential strategies to optimize the follicular environment and the IVM medium to improve the competence of oocytes during the NBS. (c) 2023 Elsevier Inc. All rights reserved.

Automated summary

The aim of this study was to evaluate the seasonal effect on the metabolomic profile of the ovarian follicle in Italian Mediterranean buffalo and identify potential strategies to improve oocyte competence during the non-breeding season. Analysis of follicular fluid, follicular cells, cumulus cells, and oocytes revealed seasonal differences in metabolite content, suggesting alterations in metabolic pathways during the non-breeding season. Pathway enrichment analysis identified metabolites linked to glutathione, energy generation, amino acid metabolism, and phospholipid biosynthesis. The identification of positive and negative competence markers in the follicular fluid provides a basis for optimizing the follicular environment and improving oocyte competence during the non-breeding season.