Knockdown of Csnk1a1 results in preimplantation developmental arrest in mice

Casein kinase 1, alpha 1 (CSNK1A1), is a member of the highly conserved serine/threonine protein kinase family. This study was established to analyze the expression and localization of CSNK1A1 and its function in early embryonic development in mice. Csnk1a1 mRNA and protein are expressed in multiple mouse tissues including the ovary. After ovulation and fertilization, Csnk1a1 mRNA and protein were detected in preimplantation embryos and their expression was highest in two-cell-stage embryos. CSNK1A1 protein was also mainly localized in the cytoplasm of preimplantation embryos. Moreover, knockdown of Csnk1a1 in zygotes led to a significant decrease in the rate of blastocyst formation. Furthermore, treat-ment of zygotes with the CSNK1A1-specific inhibitor D4476 also resulted in embryonic developmental arrest. These results provide the first evidence for a novel function of CSNK1A1 in early embryonic development in mice.(c) 2022 Elsevier Inc. All rights reserved.

Automated summary

This study investigates the expression, localization, and function of CSNK1A1 in early embryonic development in mice. CSNK1A1 is expressed in multiple mouse tissues, including the ovary, and its highest expression is observed in two-cell-stage embryos. Inhibition or knockdown of CSNK1A1 leads to a decrease in blastocyst formation and embryonic developmental arrest. These findings provide novel insights into the role of CSNK1A1 in early embryonic development.