Rapid determination of thiram on apple using a flexible bacterial cellulose-based SERS substrate

A flexible nanocomposite composed of bacterial cellulose (BC) and gold nanoparticles (AuNPs) was developed as a SERS substrate to determine thiram on apple surface by two collection methods namely paste-and-peel and wiping. Enhancement factor of this SERS substrate for sensing thiram residues was determined to be 2.8 x 105. Compared to the benchtop Raman spectrometer, portable Raman spectroscopic device showed a lower sensitivity towards thiram residues with limit of detection at 0.98 ppm, satisfying maximum residue level of thiram on apple required by both Europe and America. A good linear correlation of SERS peak intensity at 1368 cm-1 and different concentrations of thiram (1-50 ppm) revealed a coefficient up to 0.99. This flexible BC-based SERS substrate has a great analytical performance in sensitivity, reproducibility and stability, and is suitable for rapid detection (<8 min) and quantitative analysis of pesticides on food surface.

Automated summary

A flexible nanocomposite composed of bacterial cellulose (BC) and gold nanoparticles (AuNPs) was developed as a SERS substrate for the sensitive and rapid detection of thiram residues on apple surface. The SERS substrate showed a high enhancement factor and a good linear correlation, with a detection limit satisfying regulatory requirements. This BC-based SERS substrate demonstrated excellent analytical performance and is suitable for the quantitative analysis of pesticides on food surfaces.