Electrochemical sensor based on molecularly imprinted polymer cryogel and multiwalled carbon nanotubes for direct insulin detection

Insulin is the polypeptide hormone that regulates blood glucose levels. It is used as an indicator of both types of diabetes. An electrochemical insulin sensor was developed using a gold electrode modified with carboxylated multiwalled carbon nanotubes (f-MWCNTs) and molecularly imprinted polymer (MIP) cryogel. The MIP provided specific recognition sites for insulin, while the macropores of the cryogel promoted the mass transfer of insulin to the recognition sites. The f-MWCNTs increased the effective surface area and conductivity of the sensor and also reduced the potential required to oxidize insulin. Insulin oxidation was directly measured in a flow system using square wave voltammetry. This MIP cryogel/f-MWCNTs sensor provided a linear range of 0.050-1.40 pM with a very low limit of detection (LOD) of 33 fM. The sensor exhibited high selectivity and long-term stability over 10 weeks of dry storage at room temperature. The results of insulin determination in human serum using the sensor compared well with the results of the Elecsys insulin assay. The developed MIP sensor offers a promising alternative for the diagnosis and treatment of diabetes.

Automated summary

An electrochemical insulin sensor was developed using carboxylated multiwalled carbon nanotubes and molecularly imprinted polymer, which showed high sensitivity and selectivity in detecting insulin levels. The sensor offers a promising alternative for the diagnosis and treatment of diabetes.