期刊
TALANTA
卷 257, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.talanta.2023.124384
关键词
Nanopipette; G-quadruplex-hemin DNAzyme; Ion current; Hydrogen peroxide
A new sensing platform based on G-quadruplex-hemin DNAzyme (GQH) functionalized gold-coated nanopipettes was established. GQH was immobilized on the gold-coated nanopipette and acted as a catalyst for the reaction of H2O2 with ABTS to promote the conversion of ABTS to ABTS+ ions inside the nanopipette, allowing real-time monitoring of transmembrane ion current changes. The correlation between ion current and H2O2 concentration in a certain range enables the use of this immobilized nanopipette for hydrogen peroxide sensing.
Solid-nanopores/nanopipettes have the exquisite ability to reveal the changes in molecular volume due to the advantages of adjustable size, good rigidity and low noise. Herein, a new platform for sensing application was established based on G-quadruplex-hemin DNAzyme (GQH) functionalized gold-coated nanopipettes. In this method, GQH was immobilized on gold-coated nanopipette, which could be used as a catalyst for the reaction of H2O2 with 2,2 '-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) to promote the conversion of ABTS to ABTS+ ions inside gold-coated nanopipette, and the change of transmembrane ion current could be monitored in real time. At the optimal conditions, there was a correlation between the ion current and the concentration of H2O2 in a certain range, which could be used for the hydrogen peroxide sensing. The GQH immobilized nanopipette provides a useful platform to investigate enzymatic catalysis in confined environment, which can be used in electrocatalysis, sensing and fundamental electrochemistry.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据