Pseudomonas aeruginosa detection based on droplets incubation using an integrated microfluidic chip, laser spectroscopy, and machine learning

Article Chemistry, Multidisciplinary

Microfluidic Control of Coexisting Chemical Microenvironments within Multiphase Water-in-Fluorocarbon Droplets

Charles D. Crowe et al.

Summary: The use of aqueous polymer-based phase separation within water-in-oil emulsion droplets allows for control over the chemical microenvironment and the partitioning behavior of biologically relevant macromolecules.

LANGMUIR (2022)

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Article Chemistry, Analytical

Development of an on-chip microfluidic system with filter-free multiple-wavelength sensor for microflow cytometry

Tomoya Ide et al.

Summary: This study reports a compact microfluidic measurement system that can selectively identify wavelengths without using an optical component, based on a filter-free multiple-wavelength sensor. The system successfully identified two types of fluorescent beads and detected both wavelength and intensity of light using a potential peak formed by the photogate voltage.

SENSORS AND ACTUATORS B-CHEMICAL (2022)

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Article Chemistry, Multidisciplinary

On-chip classification of micro-particles using laser light scattering and machine learning

Mubashir Hussain et al.

Summary: The study proposes a method for rapid detection of microparticles using laser light scattering and support vector machines. The method shows high classification accuracy and can detect microparticles at low concentrations.

CHINESE CHEMICAL LETTERS (2022)

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Article Chemistry, Analytical

Simultaneous determination of exhaled breath vapor and exhaled breath aerosol using filter-incorporated needle-trap devices: A comparison of gas-phase and droplet-bound components

Shakiba Zeinali et al.

Summary: Breath-composition analysis is a non-invasive method for early disease diagnosis and exposure history investigation. To overcome the limitations of aerosol nature and low concentrations of volatile organic compounds in breath samples, researchers applied a needle-trap device and electrospun polyacrylonitrile filter for breath characterization. The results showed that the needle-trap device had advantages in capturing droplets and extracting free gas-phase components.

ANALYTICA CHIMICA ACTA (2022)

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Article Biophysics

SERS-based CRISPR/Cas assay on microfluidic paper analytical devices for supersensitive detection of pathogenic bacteria in foods

Jianwen Zhuang et al.

Summary: In this study, a RPA-Cas12a-mu PAD was designed using CRISPR/Cas12a and SERS technology for highly sensitive bacterial detection. The concentration of S.typhi was determined by the aggregation level of SERS nanoprobes, enabling fast and accurate testing of food samples.

BIOSENSORS & BIOELECTRONICS (2022)

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Review Biology

Dengue virus infection: A review of advances in the emerging rapid detection methods

Mubashir Hussain et al.

Summary: Dengue virus infections are increasing worldwide, posing a serious threat to children. The development of effective diagnostic systems is crucial for the progress of diagnostic tools.

BIOCELL (2022)

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Article Chemistry, Analytical

Raman spectroscopy combined with machine learning for rapid detection of food-borne pathogens at the single-cell level

Shuaishuai Yan et al.

Summary: Raman spectroscopy combined with machine learning was used to analyze single-cell spectra of common bacterial strains, achieving efficient prediction of food-borne pathogens at the serotype level.

TALANTA (2021)

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Article Chemistry, Multidisciplinary

Hand-Powered Microfluidics for Parallel Droplet Digital Loop-Mediated Isothermal Amplification Assays

Hao Yuan et al.

Summary: ddLAMP is a crucial pathogen detection technique, and the simplified version developed significantly reduces operational difficulty and equipment requirements, making it accessible even to untrained personnel.

ACS SENSORS (2021)

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Article Biochemical Research Methods

An integrated microfluidic system for early detection of sepsis-inducing bacteria

Yen-Ling Fang et al.

Summary: The study developed an integrated microfluidic chip for rapid isolation of bacteria from blood, aiding in early sepsis diagnosis. The device is efficient with a high capture rate of 85% and the entire detection process can be completed in just 4 hours.

LAB ON A CHIP (2021)

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Article Engineering, Electrical & Electronic