Colorimetric detection of biothiols and Hg2+based on the peroxidase-like activity of GTP

Guanosine-5 '-triphosphate (GTP) not only plays a key role in a majority of cellular processes but also be proposed as a peroxidase-like mimic. Compared with nanozymes, GTP shows good tolerance under harsh conditions, which can be used to construct an easy colorimetric analysis for the detection of biomolecules. Here, on the basis of the peroxidase-like activity of GTP which can catalyze the oxidation of 3,3 ',5,5 '-tetramethyl benzidine dihydrochloride (TMB), colorimetric sensing was established for biothiols and Hg2+. Biothiols reduced the oxTMB back to colorless TMB, and Hg2+ restored the formation of oxTMB, leading to the recovery of color. This method not only provides a platform for the detection of metal ions and biothiols, but also shows that GTP has great potential for analytical detection.

Automated summary

GTP not only plays a crucial role in cellular processes but also has the potential to mimic peroxidase activity. Based on its peroxidase-like activity, GTP can be used to develop a colorimetric method for detecting biomolecules. This method not only provides a platform for the detection of metal ions and biothiols, but also demonstrates the analytical potential of GTP.