期刊
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
卷 291, 期 -, 页码 -出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.saa.2022.122276
关键词
Arabidopsis thaliana; Bioimaging; Phenotyping; Light stress; Raman spectrometry; Repressor of silencing
类别
Raman spectroscopy was used to investigate the short-term (0-96 h) response of A. thaliana to oxidative stress induced by the PSII inhibitor metribuzin. Whole leaves of wildtype (WT, Col-0) and ros1 mutant were scanned, and changes in carotenoids were examined. Significant differences in Raman intensity distributions between WT and ros1 were observed, with a stronger decrease in carotenoid v1(C=C) band intensity across the leaf in ros1 after 48 h of metribuzin exposure.
The short-term (0-96 h) response of A. thaliana to the oxidative stress induced by PSII inhibitor metribuzin was examined using Raman spectroscopy. Whole leaves of wildtype (WT, Col-0) and ros1 mutant were scanned and changes in carotenoids were examined. Strong differences in Raman intensity distributions between WT and ros1 were observed. A stronger decrease of carotenoid v1(C=C) band intensity across the leaf was observed in ros1 after 48 h of exposure to metribuzin. It can be assumed that higher sensitivity to oxidative stress in ros1 mutant results in significantly faster degradation of carotenoids.
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