期刊
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
卷 292, 期 -, 页码 -出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.saa.2023.122443
关键词
Fluorescence probe; Peroxynitrite; Mitochondria; Bioimaging
类别
There is a pressing need for the development of highly sensitive and selective fluorescence probes for ONOO- in mitochondria. In this study, we introduced a ratiometric fluorescent probe COUS, which utilized a coumarin-cyanine hybrid as the fluorophore and C = C bonds as reaction sites of ONOO-. The probe COUS displayed sensitivity and selectivity towards ONOO-, with a large fluorescence emission shift (239 nm) and a low detection limit (41.88 nM). Furthermore, COUS demonstrated mitochondrial targeting ability, and the targeting moiety could dissociate from the probe upon reaction with ONOO-, enabling accurate detection of ONOO- in mitochondria.
There is an urgent need to develop highly sensitive and selective fluorescence probes for ONOO- in mitochondria. Herein, we reported a ratiometric fluorescent probe COUS with coumarin-cyanine hybrid as fluorophore and C = C bonds as reaction sites of ONOO- . The probe COUS was sensitive and selective to ONOO-, and had a large fluorescence emission shift (239 nm) as well as a low detection limit (41.88 nM). Moreover, COUS showed the mitochondrial targeting ability, and the targeting moiety could dissociate from the probe when reacting with ONOO-, which enabled COUS to accurately detect ONOO- in mitochondria.
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