4.8 Article

MXene-Based Aptameric Fluorosensor for Sensitive and Rapid Detection of COVID-19

期刊

SMALL
卷 19, 期 23, 页码 -

出版社

WILEY-V C H VERLAG GMBH
DOI: 10.1002/smll.202301146

关键词

cellular imaging; COVID-19; fluorosensors; MXene nanosheets; spike proteins

向作者/读者索取更多资源

Aptamer-functionalized MXene nanosheet is used as a novel bionanosensor for the sensitive and rapid detection of COVID-19. The sensor can detect SARS-CoV-2 spike protein and pseudovirus within 30 minutes, with high sensitivity and specificity. Its successful application for clinical sample analysis is also demonstrated.
The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)-caused COVID-19 pandemic has rapidly escalated into the largest global health emergency, which pushes to develop detection kits for the detection of COVID-19 with high sensitivity, specificity, and fast analysis. Here, aptamer-functionalized MXene nanosheet is demonstrated as a novel bionanosensor that detects COVID-19. Upon binding to the spike receptor binding domain of SARS-CoV-2, the aptamer probe is released from MXene surface restoring the quenched fluorescence. The performances of the fluorosensor are evaluated using antigen protein, cultured virus, and swab specimens from COVID-19 patients. It is evidenced that this sensor can detect SARS-CoV-2 spike protein at final concentration of 38.9 fg mL(-1) and SARS-CoV-2 pseudovirus (limit of detection: 7.2 copies) within 30 min. Its application for clinical samples analysis is also demonstrated successfully. This work offers an effective sensing platform for sensitive and rapid detection of COVID-19 with high specificity.

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.8
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

暂无数据
暂无数据