CRISPR/Cas12a coupling with RPA and MNPs for rapid and visualized identification of methicillin-resistant Staphylococcus aureus

Article Chemistry, Analytical

Strand Displacement Amplification Assisted CRISPR-Cas12a Strategy for Colorimetric Analysis of Viral Nucleic Acid

Shaohua Gong et al.

Summary: The SDACC method reported in this study enables sensitive detection of viral nucleic acid, providing a simple and low-cost approach for colorimetric analysis.

ANALYTICAL CHEMISTRY (2021)

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Article Chemistry, Multidisciplinary

CRISPR-Cas12a-Based Nucleic Acid Amplification-Free DNA Biosensor via Au Nanoparticle-Assisted Metal-Enhanced Fluorescence and Colorimetric Analysis

Jin-Ha Choi et al.

Summary: A non-nucleic acid amplification fluorescent biosensor using gold nanoparticles for metal-enhanced fluorescence detection of cfDNA has been developed in this study, allowing rapid and highly sensitive detection of BRCA-1. This sensor is highly selective and can be widely applied for the measurement of other nucleic acid biomarkers.

NANO LETTERS (2021)

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Article Chemistry, Analytical

Ultrasensitive microchip electrophoretic detection of the mecA gene in methicillin-resistant Staphylococcus aureus (MRSA) based on isothermal strand-displacement polymerase reaction

Yuqi Lu et al.

Summary: A method integrating ISDPR with MCE was developed for rapid and sensitive detection of the mecA gene in methicillin-resistant Staphylococcus aureus strains. The method showed advantages such as label-free detection, ultra-sensitivity, rapid detection, and efficient separation of amplified products.

TALANTA (2021)

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Article Chemistry, Multidisciplinary

Detection of SARS-CoV-2 by CRISPR/Cas12a-Enhanced Colorimetry

Yongzhong Jiang et al.

Summary: The outbreak of COVID-19 led to a global public health crisis, and large-scale population screening is an effective means to control its spread. Scientists have developed a magnetic pull-down-assisted colorimetric method based on the CRISPR/Cas12a system (M-CDC) for rapid and accurate detection of SARS-CoV-2, without the need for expensive instruments.

ACS SENSORS (2021)

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Article Chemistry, Multidisciplinary

Diagnosing Antibiotic Resistance Using Nucleic Acid Enzymes and Gold Nanoparticles

Mohamed A. Abdou Mohamed et al.

Summary: The study developed a rapid, sensitive, and simple colorimetric nanodiagnostic platform for identifying disease-causing pathogens and their associated antibiotic resistance genes within 2 hours. The platform demonstrated high clinical sensitivity and specificity in detecting bacteria and antibiotic resistance markers from different biological samples, making it attractive for broad screening of microbial pathogens.

ACS NANO (2021)

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Article Chemistry, Analytical

Universal and Programmable Rolling Circle Amplification-CRISPR/Cas12a-Mediated Immobilization-Free Electrochemical Biosensor

Min Qing et al.

Summary: The study introduced the CRISPR/Cas system into an electrochemical biosensing platform for sensitive and specific detection of disease-related molecules. By utilizing modular rolling circle amplification and CRISPR/Cas12a, signal amplification was achieved. The flexibility and programmability of this strategy allow for the detection of various targets.

ANALYTICAL CHEMISTRY (2021)

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Article Chemistry, Analytical

Multivalent Duplexed-Aptamer Networks Regulated a CRISPRCas12a System for Circulating Tumor Cell Detection

Zhengxian Lv et al.

Summary: A CRISPR-Cas12a-based CTC detection sensor regulated by MDANs and a 3D DNA network formed by RCA products showed improved sensitivity for detecting CTCs. The method was successfully applied to detect CTCs in human blood samples, indicating great potential for clinical CTC-based liquid biopsy.

ANALYTICAL CHEMISTRY (2021)

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Article Chemistry, Multidisciplinary

Droplet Cas12a Assay Enables DNA Quantification from Unamplified Samples at the Single-Molecule Level

Huahua Yue et al.

Summary: This study presents a CRISPR-Cas12a-based molecular diagnostic technique for amplification-free and absolute quantification of DNA at the single-molecule level, achieving high sensitivity and specificity through optimized reaction parameters and microdroplet technology. The method can directly count diverse viruses' DNAs and demonstrate versatility and quantification capability, showing potential as a versatile and quantitative platform for molecular diagnosis.

NANO LETTERS (2021)

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Article Chemistry, Multidisciplinary

CRISPR-Cas12a-Powered Dual-Mode Biosensor for Ultrasensitive and Cross-validating Detection of Pathogenic Bacteria

Long Ma et al.

Summary: The study proposed a CRISPR-Cas12a-powered dual-mode biosensor for ultrasensitive and cross-validating detection of pathogenic bacteria, with satisfactory selectivity for Salmonella and capabilities in food sample detection. The technology expands the reach of CRISPR-Cas system in biosensing and provides a general method for bacteria sensing with desirable sensitivity, specificity, and cross-validating capacity.

ACS SENSORS (2021)

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Review Engineering, Biomedical

CRISPR-based diagnostics

Michael M. Kaminski et al.

Summary: This Review summarizes the rapidly expanding toolbox of CRISPR-based diagnostics, highlighting their applications in real-time diagnosis. It mentions that nucleic acid-based diagnostics are sensitive and specific, but most require expensive equipment and trained personnel.

NATURE BIOMEDICAL ENGINEERING (2021)

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Article Chemistry, Multidisciplinary