期刊
SENSORS AND ACTUATORS B-CHEMICAL
卷 380, 期 -, 页码 -出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2023.133373
关键词
Fluorescence resonance energy transfer; Alzheimer?s disease; Strand displacement amplification; Rolling circle amplification
This study developed quadruple cascade strand displacement amplification (QC-SDA)-mediated polyfluorene derivative-based multiple fluorescence resonance energy transfer (FRET) for simultaneous determination of AD biomarkers miRNA-16 and amyloid-beta oligomer species (A beta Os). The method achieved high-throughput detections with high sensitivity and ease of operation.
Currently reported detections for biomarkers of Alzheimer's disease (AD) were usually single-target detections, which were prone to generate misdiagnosis. This work developed quadruple cascade strand displacement amplification (QC-SDA)-mediated polyfluorene derivative-based multiple fluorescence resonance energy transfer (FRET) for simultaneous determination of AD biomarkers miRNA-16 and amyloid-beta oligomer species (A beta Os). Carboxyl-functionalized poly [9,9-bis(3 '-(N,N-dimethylamino)propyl)- 2,7-fluorene]-alt-2,7-(9,9-dioctyl-fluorene)] polymer nanoparticles (c-PFN PNPs) served as fluorescent donor and two fluorescent dyes served as fluorescent acceptors. When the targets combined with templates to continuously trigger QC-SDA, multiple infinite cycles occurred to generate numerous triggers. The resulting triggers initiated rolling circle amplification (RCA) to generate repeated sequences, which further hybridized with helper probes and reporter probes to achieve substantial immobilization of fluorescent acceptors on c-PFN PNPs, thereby realizing multiple FRET from c-PFN PNPs to acceptors with only single excitation. As FAM and Texas Red corresponded to miRNA-16 and A beta Os, the high-throughput detections of biomarkers were achieved with limit of detection of 110 aM and 1.3 fM, respectively. The c-PFN PNPs coupling FAM and Texas Red provided an ideal multiplex FRET platform for synchronous detection of multi-target. What's more, the creative incorporation of amplification strategies QC-SDA and RCA endowed multiplex FRET with high sensitivity and ease of operation for bioanalysis.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据