Neurotransmitter disturbances caused by methylmercury exposure: Microbiota-gut-brain interaction

The fetal and early postnatal stages are periods of rapid brain development, during which, methylmercury (MeHg) ex-posure can cause lasting cognitive impairments. MeHg exposure disrupts neurotransmitter metabolites, which in-creased susceptibility to neurological responses. However, the neurotoxic mechanism underlying the MeHg-induced disruption of neurotransmitter metabolism requires further exploration. To this end, female Sprague-Dawley (SD) rats were administered NaCl (control group) or MeHg (0.6 mg/kg, 1.2 mg/kg and 2.4 mg/ kg body weight (bw), where the body weight refers to the dams) during the perinatal period, and then changes in neurotransmitter profiles and the gut microbiota of offspring were detected. The results showed that tryptophan (Trp) and tyrosine (Tyr) path-way neurotransmitter metabolites, including serotonin (5-HT), 5-hydroxy indole acetic acid (5-HIAA), N-acetyl-5-hydroxytryptamin (NAS), Tyr, dopamine (DA) and epinephrine (E), were significantly changed, and the Kynurenine/Tryptophan (Kyn/Trp) ratio was increased in the MeHg-treated groups. Meanwhile, acetylcholine (ACh) and neurotransmitters involved in the amino acid pathway were significantly reduced. Notably, MeHg treat-ment induced a significant reduction in tight junctions in the colon and hippocampal tissue. Furthermore, fecal microbiota analysis indicated that the diversity and composition characteristics were significantly altered by MeHg exposure. Mediation analysis showed that the gut microbiota mediated the effect of MeHg treatment on the neuro-transmitter expression profiles. The present findings shed light on the regulatory role of the gut microbiota in MeHg-disrupted neurotransmitter metabolic pathways and the potential impact of perinatal MeHg treatment on the cross-talk between the gut and brain.

Automated summary

The fetal and early postnatal stages are crucial periods for brain development, but exposure to methylmercury (MeHg) during this time can lead to long-lasting cognitive impairments. This study investigated the neurotoxic mechanism of MeHg in disrupting neurotransmitter metabolism and found that MeHg significantly altered neurotransmitter profiles and the gut microbiota in offspring rats. The findings indicate a potential regulatory role of the gut microbiota in MeHg-induced disruptions and suggest the impact of perinatal MeHg exposure on gut-brain communication.