Investigation of changes in serum thiols and neutrophil-to-lymphocyte ratio, monocyte-to-lymphocyte ratio, platelet-to-lymphocyte ratio, and mean platelet volume/platelet count ratio indices in cats undergoing ovariohysterectomy

The aim of this study was to investigate complete blood indices [neutrophil-to-lymphocyte ratio (NLR), monocyte-to-lymphocyte ratio (MLR), platelet-to-lymphocyte ratio (PLR) and mean platelet volume/platelet count ratio (MPV/PLT)] and thiols (native and thiol) in cats undergoing ovariohysterectomy (OHE). The study sample comprised a total of 10 female cats of mixed breeds. Ovariohysterectomy operation was performed under Ketamine/ Xylazine anesthesia with the appropriate technique. Blood samples were collected from the cephalic vein pre-OHE and post-OHE at 2 h, 24 h, and 7 d. Total and native thiol levels in the serum were analyzed using a colorimetric technique, and whole blood analysis was carried out using anticoagulant tubes. White Blood Cell (WBC) and neutrophil increased significantly at 2 h post-OHE, peaked at 24 h post-OHE, then returned to baseline levels by 7 d (P<0.001). The lymphocyte and thiols decreased at 2 h, 24 h, and 7 d post-OHE, and the lowest lymphocyte count was observed at 24 h (P<0.001). The lowest monocyte count was observed on d 7 post-OHE (P<0.001). NLR increased significantly 2 h after OHE, peaked at 24 h after OHE, and remained high on d 7 (P<0.001). MLR increased at 2 h, peaked at 24 h, then returned to baseline levels on d 7 (P<0.001). PLR increased gradually and peaked at 24 h and on d 7 (P<0.001). MPV/PLT after OHE was statistically lowest on d 7 (P<0.05). A negative correlation was determined between NLR, MLR, PLR, and thiols (P<0.001). In conclusion, thiols and complete blood indices (NLR, MLR, and PLR) may be important in the assessment of inflammation and stress responses after OHE in cats.

Automated summary

The aim of this study was to investigate the changes in complete blood indices and thiols in cats undergoing ovariohysterectomy (OHE). The results showed that neutrophil-to-lymphocyte ratio (NLR), monocyte-to-lymphocyte ratio (MLR), and platelet-to-lymphocyte ratio (PLR) increased significantly after OHE, while lymphocyte count and thiols decreased. The findings suggest that these indices and thiols may play an important role in assessing inflammation and stress responses after OHE in cats.