Ginkgo, fennel, and flaxseed can affect hormone release by porcine ovarian cells and modulate the effect of toluene

Experimental studies have documented the toxic effects of toluene on the mammalian female reproductive processes. The aim of this in vitro study was to examine the potential of functional food plant extracts, namely, of ginkgo, fennel, and flaxseed, in modifying the toluene-induced effects on ovarian hormone release. Porcine granulosa cells were incubated with ginkgo, fennel, or flaxseed extracts (0, 1, 10, or 100 mu g/mL) and/or toluene (10 mu g/mL). Enzyme immunoassays were used in order to measure the release of progesterone (P), oxytocin (OT), and prostaglandin F (PGF) in the culture media. Toluene suppressed the release of P and enhanced the release of OT and PGF. All tested plant extracts reduced P and increased OT release, while the PGF output was found inhibited by ginkgo and stimulated by fennel and flaxseed. When the cells were incubated with toluene and each one of the plant extracts, toluene was able to prevent their action on P release, as well as those of fennel and flaxseed on OT and PGF release. Moreover, ginkgo enhanced but fennel or flaxseed prevented the toluene -induced effects on OT and PGF release. These observations (i) document novel aspects of the toluene-induced toxicity; (ii) demonstrate the direct influence of ginkgo, fennel, and flaxseed extracts on the ovarian secretory activity; (iii) inform our understanding of the interrelationship between toluene and the tested plant extracts with regard to their effects on ovarian hormone release; (iiii) demonstrate the ability of fennel and flaxseed to prevent adverse effect of toluene on ovarian hormones.

Automated summary

Experimental studies have found that toluene has toxic effects on female reproductive processes. This in vitro study aimed to investigate the potential of ginkgo, fennel, and flaxseed extracts to modify the effects of toluene on ovarian hormone release. The extracts were found to reduce progesterone release and increase oxytocin release, while the release of prostaglandin F was inhibited by ginkgo and stimulated by fennel and flaxseed. Furthermore, fennel and flaxseed were able to prevent the adverse effects of toluene on ovarian hormones, while ginkgo enhanced these effects.